Evaluation of magnesium efficiency in natural populations of oilseed rape at stage of seedling and screening of elite germplasm with high magnesium efficiency

XU Wenying; LIU Liang; ZHANG Kun; ZHANG Haiwei; XIAO Qingliang; WU Ketian; YANG Guangzhe; ZHAO Zunkang

doi:10.13300/j.cnki.hnlkxb.2025.05.007

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Evaluation of magnesium efficiency in natural populations of oilseed rape at stage of seedling and screening of elite germplasm with high magnesium efficiency

Accurate evaluation and identification of crop varieties resistant to barrenness and abiotic stress | 更新时间：2026-11-05

- Evaluation of magnesium efficiency in natural populations of oilseed rape at stage of seedling and screening of elite germplasm with high magnesium efficiency
- Journal of Huazhong Agricultural University Vol. 44, Issue 5, Pages: 69-79(2025)
- 作者机构：
  
  1.江西农业大学国土资源与环境学院/江西省农业废弃物资源化利用与面源污染防控产教融合重点创新中心，南昌330045
  2.江西省植保植检站，南昌 330096
  3.江西省烟草科学研究所，南昌 330025
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.05.007
  CLC： S565.4
- Received：07 July 2025，
  
  Published：15 September 2025
- 稿件说明：
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徐文英，刘良，张昆，等.甘蓝型油菜自然群体苗期镁效率评价及镁高效优异种质筛选［J］.华中农业大学学报，2025，44（5）：69‐79.

XU Wenying,LIU Liang,ZHANG Kun,et al.Evaluation of magnesium efficiency in natural populations of oilseed rape at stage of seedling and screening of elite germplasm with high magnesium efficiency[J].Journal of Huazhong Agricultural University,2025,44(05):69-79.
徐文英，刘良，张昆，等.甘蓝型油菜自然群体苗期镁效率评价及镁高效优异种质筛选［J］.华中农业大学学报，2025，44（5）：69‐79. DOI： 10.13300/j.cnki.hnlkxb.2025.05.007.

XU Wenying,LIU Liang,ZHANG Kun,et al.Evaluation of magnesium efficiency in natural populations of oilseed rape at stage of seedling and screening of elite germplasm with high magnesium efficiency[J].Journal of Huazhong Agricultural University,2025,44(05):69-79. DOI： 10.13300/j.cnki.hnlkxb.2025.05.007.

摘要

为筛选甘蓝型油菜镁高效品种，研究油菜镁高效分子机制，以甘蓝型油菜自然群体90个品种为材料，采用苗期营养液培养两步筛选法，考查群体在低镁和镁正常处理下鲜物质量、干物质量、根冠比和镁累积量等10个性状，利用相关性分析、聚类分析、综合评价分析法对90个品种进行镁效率的综合评价并筛选镁高效品种。结果显示，90个油菜品种在2个镁处理下10个性状均呈显著差异，变异系数为14.8%~82.94%，且10个性状的耐低镁系数均呈显著正相关；以镁正常条件下干物质生物量和镁效率系数为主要评价指标，将90个品种分为4个类型，分别为高效低敏感型（14个）、高效高敏感型（21个）、低效高敏感型（19个）和低效低敏感型（36个）。通过复筛验证最终筛选到甘蓝型油菜镁高效品种W16与W100和镁低效品种W61与W132，可为后续油菜镁高效分子机制研究提供材料。

Abstract

Screening magnesium （Mg）-efficient germplasm of oilseed rape （

Brassica napus

L.） will provide elite materials for studying molecular mechanism of Mg efficiency in

Brassica napus

.90 varieties of natural population of

B. napus

were used to examine 10 traits of the population including the weight of fresh biomass，the weight of dry biomass，the ratio of root to shoot and the accumulation of Mg under low and normal Mg treatments with the two-step screening method of nutrient solution culture at the stage of seedling to screen magnesium efficient varieties of

B. napus

and study the molecular mechanism of magnesium efficiency in

B. napus

.Correlation analysis，cluster analysis，and comprehensive evaluation analysis methods were used to comprehensively evaluate the magnesium efficiency in 90 varieties and screen magnesium efficient varieties.The results showed that there were significant differences in 10 traits of 90 varieties under 2 magnesium treatments，with a coefficient of variation ranging from 14.8% to 82.94%.The coefficient of low-Mg tolerance of 10 traits was significantly positively correlated.90 varieties were divided into four types including high-efficiency and low sensitivity type （14 varieties），high-efficiency and high sensitivity type （21 varieties），low efficiency and high sensitivity type （19 varieties），and low efficiency and low sensitivity type （36 varieties） based on the weight of dry biomass and the coefficient of magnesium efficiency under normal magnesium conditions as the main indexes for evaluation.The magnesium efficient varieties including W16 and W100 and magnesium inefficient varieties including W61 and W132 of

B. napus

were finally screened out through re-screening verification，which can provide materials for subsequent studies on the molecular mechanism of high magnesium efficiency in

B. napus

关键词

Keywords

references

CHEN Z C，PENG W T，LI J，et al.Functional dissection and transport mechanism of magnesium in plants［J］.Seminars in cell & developmental biology，2018，74：142-152.

MARCHNER H，MARSCHNER P.Marschne’s mineral nutrition of higher plants［M］.London：Waltham Academic Press，2012.

DE BANG T C，HUSTED S，LAURSEN K H，et al.The molecular-physiological functions of mineral macronutrients and their consequences for deficiency symptoms in plants［J］.New phytologist，2021，229（5）：2446-2469.

TRÄNKNER M，TAVAKOL E，JÁKLI B.Functioning of potassium and magnesium in photosynthesis，photosynthate translocation and photoprotection［J/OL］.Physiologia plantarum，2018：12747［2025-07-07］.https：//doi.org/10.1111/ppl.12747.

JAMALI JAGHDANI S，JAHNS P，TRÄNKNER M.Mg deficiency induces photo-oxidative stress primarily by limiting CO 2 assimilation and not by limiting photosynthetic light utilization［J/OL］.Plant science，2021，302：110751［2025-07-07］.https：//doi.org/10.1016/j.plantsci.2020.110751.

RUDE R K，SINGER F R，GRUBER H E.Skeletal and hormonal effects of magnesium deficiency［J］.Journal of the American college of nutrition，2009，28（2）：131-141.

GUO W L，NAZIM H，LIANG Z S，et al.Magnesium deficiency in plants：an urgent problem［J］.The crop journal，2016，4（2）：83-91.

中华人民共和国国家统计局.中国统计年鉴［M］.北京：中国统计出版社，2021.National Bureau of Statistics of the People’s Republic of China.China statistical yearbook ［M］.Beijing： China Statistics Press，2021 （in Chinese） .

邹娟，鲁剑巍，吴江生，等.4个双低甘蓝型油菜品种钙、镁、硫吸收动态［J］.华中农业大学学报，2009，28（3）：295-299.ZOU J，LU J W，WU J S，et al.Dynamics of calcium，magnesium and sulfur uptake in 4 double-low rapeseed（ Brassica napus L.） varieties［J］.Journal of Huazhong Agricultural University，2009，28（3）：295-299 （in Chinese with English abstract） .

任涛，郭丽璇，张丽梅，等.我国冬油菜典型种植区域土壤养分现状分析［J］.中国农业科学，2020，53（8）：1606-1616.REN T，GUO L X，ZHANG L M，et al.Soil nutrient status of oilseed rape cultivated soil in typical winter oilseed rape production regions in China［J］.Scientia agricultura sinica，2020，53（8）：1606-1616 （in Chinese with English abstract） .

ZHANG L D，PENG Y Y，LI J，et al.OsMGT1 confers resistance to magnesium deficiency by enhancing the import of Mg in rice［J/OL］.International journal of molecular sciences，2019，20（1）：207［2025-07-07］.https：//doi.org/10.3390/ijms20010207.

ZHANG B，ZHANG C，TANG R J，et al.Two magnesium transporters in the chloroplast in ner envelope essential for thylakoid biogenesis in Arabidopsis ［J］.New phytologist，2022，236（2）：464-478.

徐永成.全基因组关联分析发掘水稻镁高效吸收遗传变异位点和材料［D］.南京：南京农业大学，2020.XU Y C.Genome-wide association study excavated genetic variation sites and materials with high magnesium absorption efficiency in rice［D］.Nanjing：Nanjing Agricultural University，2020 （in Chinese with English abstract） .

ANWAR A，AKHTAR J，ALEEM S，et al.Genome-wide identification of MGT gene family in soybean （ Glycine max） and their expression analyses under magnesium stress conditions［J/OL］.BMC plant biology，2025，25（1）：83［2025-07-07］.https：//doi.org/10.1186/s12870-024-05985-7.

REGON P，CHOWRA U，AWASTHI J P，et al.Genome-wide analysis of magnesium transporter genes in Solanum lycopersicum ［J］.Computational biology and chemistry，2019，80：498-511.

WANG Y J，HUA X T，XU J S，et al.Comparative genomics revealed the gene evolution and functional divergence of magnesium transporter families in saccharum［J/OL］.BMC genomics，2019，20（1）：83［2025-07-07］.https：//doi.org/10.1186/s12864-019-5437-3.

LI H Y，WANG N，DING J Z，et al.The maize CorA /MRS2/MGT-type Mg transporter，ZmMGT10，responses to magnesium deficiency and confers low magnesium tolerance in transgenic Arabidopsis ［J］.Plant molecular biology，2017，95（3）：269-278.

MENG S F，ZHANG B，TANG R J，et al.Four plasma membrane-localized MGR transporters mediate xylem Mg 2+ loading for root-to-shoot Mg 2+ translocation in Arabidopsis ［J］.Molecular plant，2022，15（5）：805-819.

SAITO T，KOBAYASHI N I，TANOI K，et al.Expression and functional analysis of the CorA -MRS2-ALR-type magnesium transporter family in rice［J］.Plant & cell physiology，2013，54（10）：1673-1683.

LI J，YOKOSHO K，LIU S，et al.Diel magnesium fluctuations in chloroplasts contribute to photosynthesis in rice［J］.Nature plants，2020，6（7）：848-859.

SUN J J，LI S T，WANG S F，et al.Characterization of the main magnesium transporters mediating different Mg translocation from root to shoot between Mg-tolerant and Mg-sensitive Brassica napus cultivars under magnesium deficiency stress［J］.Plant and soil，2019，445（1）：453-468.

陆志峰，任涛，鲁剑巍.我国冬油菜种植区土壤有效镁状况与油菜施镁效果［J］.华中农业大学学报，2021，40（2）：17-23.LU Z F，REN T，LU J W.Soil available magnesium status and effects of magnesium application on rapeseed yield in main producing area of China［J］.Journal of Huazhong Agricultural University，2021，40（2）：17-23 （in Chinese with English abstract） .

白由路，金继运，杨俐苹.我国土壤有效镁含量及分布状况与含镁肥料的应用前景研究［J］.土壤肥料，2004（2）：3-5. BAI Y L，JIN J Y，YANG L P.Study on the content and distribution of soil available magnesium and foreground of magnesium fertilizer in China［J］.Soils and fertilizers，2004（2）：3-5 （in Chinese with English abstract） .

MAGUIRE M E，COWAN J A.Magnesium chemistry and biochemistry［J］.Biometals，2002，15（3）：203-210.

SENBAYRAM M，GRANSEE A，WAHLE V，et al.Role of magnesium fertilisers in agriculture：plant–soil continuum［J］.Crop and pasture science，2015，66（12）：1219-1229.

楮海燕，喻敏，王运华，等.甘蓝型油菜品种硼利用效率的差异研究［J］.华中农业大学学报，1999，18（2）：38-42.CHU H Y，YU M，WANG Y H，et al.Study on the difference of boron utilization efficiency of Brassica napus varieties［J］.Journal of Huazhong Agricultural University，1999，18（2）：38-42 （in Chinese with English abstract） .

刘波，魏全全，鲁剑巍，等.苗期渍水和氮肥用量对直播冬油菜产量及氮肥利用率的影响［J］.植物营养与肥料学报，2017，23（1）：144-153.LIU B，WEI Q Q，LU J W，et al.Effects of waterlogging at the seedling stage and nitrogen application on seed yields and nitrogen use efficiency of direct-sown winter rapeseed（ Brassica napus L.）［J］.Journal of plant nutrition and fertilizer，2017，23（1）：144-153 （in Chinese with English abstract） .

张海伟，黄宇，叶祥盛，等.甘蓝型油菜重组自交系苗期磷效率的评价［J］.作物学报，2008，34（12）：2152-2159.ZHANG H W，HUANG Y，YE X S，et al.Evaluation of phosphorus efficiency in rapeseed （ Brassica napus L.） recombinant inbred lines at seedling stage［J ］.Acta agronomica sinica，2008，34（12）：2152-2159 （in Chinese with English abstract） .

张雯，黄益丹，张鹏超，等.甘蓝型油菜耐铝极端品种筛选及耐铝生理机制初步解析［J］.华中农业大学学报，2023，42（6）： 154-163.ZHANG W，HUANG Y D，ZHANG P C，et al.Screening extreme varieties with aluminum tolerance and analyzing physiological mechanisms of aluminum tolerance in Brassica napus ［J］.Journal of Huazhong Agricultural University，2023，42（6）： 154-163 （in Chinese with English abstract） .

WU D Z，LIANG Z，YAN T，et al.Whole-genome resequencing of a worldwide collection of rapeseed accessions reveals the genetic basis of ecotype divergence［J］.Molecular plant，2019，12（1）：30-43.

张恒，陈艳琦，任杰莹，等.西南麦区小麦苗期氮高效品种筛选及指标体系构建［J］.四川农业大学学报，2022，40（1）：10-18.ZHANG H，CHEN Y Q，REN J Y，et al.Screening of wheat cultivars with high nitrogen efficiency at seedling stage and construction of index system in southwest wheat region［J］.Journal of Sichuan Agricultural University，2022，40（1）：10-18 （in Chinese with English abstract） .

连盈，卢娟，胡成梅，等.低氮胁迫对谷子苗期性状的影响和耐低氮品种的筛选［J］.中国生态农业学报（中英文），2020，28（4）：523-534.LIAN Y，LU J，HU C M，et al.Effects of low nitrogen stress on foxtail millet seedling characteristics and screening of low nitrogen tolerant varieties［J］.Chinese journal of eco-agriculture，2020，28（4）：523-534 （in Chinese with English abstract） .

付鸾鸿，于崧，于立河，等.不同基因型燕麦萌发期耐盐碱性分析及其鉴定指标的筛选［J］.作物杂志，2018（6）：27-35.FU L H，YU S， YU L H，et al.Analysis of saline-alkaline tolerance and screening of identification indexes of different oat genotypes at the germination stage［J］.Crops，2018（6）：27-35 （in Chinese with English abstract） .

于崧，郭潇潇，梁海芸，等.不同基因型绿豆萌发期耐盐碱性分析及其鉴定指标的筛选［J］.植物生理学报，2017，53（9）：1629-1639.YU S，GUO X X，LIANG H Y，et al.Analysis of saline-alkaline tolerance and screening of identification indicators at the germination stage among different mung bean genotypes［J］.Plant physiology journal，2017，53（9）：1629-1639 （in Chinese with English abstract） .

DUAN H Y，SHI L，YE X S，et al.Identification of phosphorous efficient germplasm in oilseed rape［J］.Journal of plant nutrition，2009，32（7）：1148-1163.

陈燕玲，王宁，石磊.甘蓝型油菜自然群体苗期磷效率分析及磷高效优异种质筛选［J］.中国油料作物学报，2023，45（1）：56-62.CHEN Y L，WANG N，SHI L.Analysis of phosphorus efficiency and screening of P-efficient germplasm on natural population of oilseed rape （ Brassica napus ） at seedling stage［J］.Chinese journal of oil crop sciences，2023，45（1）：56-62 （in Chinese with English abstract） .

张鹏超，张雯，黄益丹，等.甘蓝型油菜苗期耐酸性综合评价及耐酸种质筛选［J］.华中农业大学学报，2023，42（6）： 164-174.ZHANG P C，ZHANG W，HUANG Y D，et al.Comprehensive evaluation of acid-tolerance and screening of acid-tolerant germplasm in Brassica napus at seedling stage ［J］.Journal of Huazhong Agricultural University，2023，42（6）： 164-174 （in Chinese with English abstract） .

夏龙飞，易科，张木清，等.甘蔗磷效率基因型差异及磷高效基因型筛选研究［J］.热带作物学报，2017，38（5）：873-880.XIA L F，YI K，ZHANG M Q，et al.Genotypic differences of phosphorus efficiency and screening of phosphorus efficient cultivars in sugarcane［J］.Chinese journal of tropical crops，2017，38（5）：873-880 （in Chinese with English abstract） .

徐子先.甘蓝型油菜氮效率评价及其差异的生理机制探究［D］.北京：中国农业科学院，2017.XU Z X.Evaluation of nitrogen efficiency in Brassica napus and physiological mechanism of its differences［D］.Beijing：Chinese Academy of Agricultural Sciences，2017 （in Chinese with English abstract） .

廖博.镁营养缺乏对油菜叶片同化物积累的影响及其生理机制［D］.武汉：华中农业大学，2024.LIAO B.Effect of magnesium deficiency on the accumulation of assimilates in oilseed rape leaves and its physiological mechanisms ［D］.Wuhan：Huazhong Agricultural University，2024 （in Chinese with English abstract） .

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