Effects of nitrogen application rate on agronomic traits of <italic style="font-style: italic">Brassica napus</italic> L. and screening of nitrogen-efficient genotypes

REN Lulu; LI Shuang; ZHANG Hao; WANG Dandan; WANG Chuang; SHI Lei; XU Fangsen; DING Guangda

doi:10.13300/j.cnki.hnlkxb.2025.05.006

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Effects of nitrogen application rate on agronomic traits of Brassica napus L. and screening of nitrogen-efficient genotypes

Accurate evaluation and identification of crop varieties resistant to barrenness and abiotic stress | 更新时间：2026-11-05

- Effects of nitrogen application rate on agronomic traits of Brassica napus L. and screening of nitrogen-efficient genotypes
- Journal of Huazhong Agricultural University Vol. 44, Issue 5, Pages: 60-68(2025)
- 作者机构：
  
  华中农业大学资源与环境学院/微量元素研究中心/农业农村部长江中下游耕地保育重点实验室，武汉 430070
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.05.006
  CLC： S565.4
- Received：17 July 2025，
  
  Published：15 September 2025
- 稿件说明：
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任璐璐，李双，张浩，等.施氮水平对甘蓝型油菜农艺性状的影响及氮高效基因型筛选［J］.华中农业大学学报，2025，44（5）：60‐68.

REN Lulu,LI Shuang,ZHANG Hao,et al.Effects of nitrogen application rate on agronomic traits of Brassica napus L. and screening of nitrogen-efficient genotypes[J].Journal of Huazhong Agricultural University,2025,44(05):60-68.
任璐璐，李双，张浩，等.施氮水平对甘蓝型油菜农艺性状的影响及氮高效基因型筛选［J］.华中农业大学学报，2025，44（5）：60‐68. DOI： 10.13300/j.cnki.hnlkxb.2025.05.006.

REN Lulu,LI Shuang,ZHANG Hao,et al.Effects of nitrogen application rate on agronomic traits of Brassica napus L. and screening of nitrogen-efficient genotypes[J].Journal of Huazhong Agricultural University,2025,44(05):60-68. DOI： 10.13300/j.cnki.hnlkxb.2025.05.006.

摘要

为筛选甘蓝型油菜氮高效品种以降低氮肥依赖、推动绿色生产，采用梯度氮处理（氮肥用量分别为：高氮210 kg/hm²、低氮70 kg/hm²、不施氮0 kg/hm²）对14个甘蓝型油菜基因型进行田间试验，测定农艺性状、籽粒产量及氮效率相关指标：氮肥偏生产力（partial factor productivity of nitrogen，PFPN）、氮肥农学效率（agronomic efficiency of nitrogen， AEN），结合农艺性状相关性分析及氮效率类型进行氮效率综合评价。结果显示：低氮胁迫下，不同基因型油菜对氮素的响应差异更显著，氮效率指标表现出明显分化，PFPN与AEN均值分别达到高氮处理的2.75倍和2.30倍；筛选出双高效型基因型，即在高氮和低氮处理下，籽粒产量均显著高于所有供试基因型在对应氮水平的平均产量，PFPN与AEN稳定优异，在高低氮条件下均保持较高水平（高氮下PFPN≥12 kg/kg、AEN≥5 kg/kg；低氮下 PFPN≥30 kg/kg、AEN≥10 kg/kg）；低氮高效型在低氮下PFPN达50.61 kg/kg，AEN达43.92 kg/kg；高氮高效型仅在高氮表现突出；氮素调控农艺性状相关性，施氮强化地上部干质量、角果数与产量的正相关关系；双高效型占品种总数的28.57%，其生物量积累稳定；低氮高效型通过提升千粒重和每角果粒数适应氮胁迫。综上，双高效型品种和低氮高效型品种可为油菜氮高效遗传改良及减肥增效栽培提供重要种质资源。

Abstract

A Field experiment was conducted on 14 genotypes of

Brassica napus

L. with gradient nitrogen treatments including nitrogen fertilizer application rates of high nitrogen 210 kg/hm²， low nitrogen 70 kg/hm²， and no nitrogen application 0 kg/hm² to screen nitrogen-efficient varieties of

B. napus

to reduce the dependence on nitrogen fertilizer and promote the green production. Agronomic traits， seed yield， and nitrogen efficiency related indexes including partial factor productivity of nitrogen （PFPN） and agronomic efficiency of nitrogen （AEN） were measured. The nitrogen efficiency was comprehensively evaluated based on correlation analysis of agronomic traits and classification of nitrogen efficiency types. The results showed that there were more significant differences in the response of different genotypes of

B. napus

L. to nitrogen under the low nitrogen treatment， with significant differentiation in nitrogen efficiency related indexes. The mean value of PFPN and AEN reached 2.75 times and 2.30 times higher than that under the high nitrogen treatment， respectively. The dual highly-efficient genotypes of

B.napus

L. with significantly higher seed yield than the average yield of all genotypes t

ested at corresponding nitrogen rate under the high and low nitrogen treatments were screened. PFPN and AEN were stable and excellent， maintaining high levels under the high and low nitrogen treatments （PFPN≥12 kg/kg， AEN ≥5 kg/kg under the high nitrogen treatment； PFPN≥30 kg/kg， AEN≥10 kg/kg under the low nitrogen treatment）. The low nitrogen-efficient genotypes achieved a PFPN of 50.61 kg/kg and an AEN of 43.92 kg/kg under the low nitrogen treatment. The high nitrogen-efficient genotypes performed prominently only under the high nitrogen treatment. Nitrogen application regulated correlations among agronomic traits， with enhanced the positive correlations between aboveground dry weight， number of siliques， and seed yield under nitrogen application. The proportion of dual highly-efficient genotypes was 28.57%， with stable accumulation of biomass. The low nitrogen-efficient genotypes adapted to the low nitrogen treatment by exhibiting high thousand-seed weight and seeds per silique. It is indicated that dual highly-efficient and low nitrogen-efficient varieties of

B. napus

L. can provide valuable germplasm for the genetic improvement of nitrogen use efficiency and the development of cultivation strategies to reduce fertilizer and improve efficiency in

B. napus

L..

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Related Author

DING Guangda

XU Fangsen

SHI Lei

WANG Chuang

WANG Dandan

ZHANG Hao

LI Shuang

REN Lulu

Related Institution

College of Horticulture and Forestry Sciences，Huazhong Agricultural University

Hubei Xianning Academy of Agriculture Sciences

Shandong Quanlu Kerun Seed Co.， Ltd.， Zhucheng

National Key Laboratory of Crop Genetic Improvement/Hubei Hongshan Laboratory， Huazhong Agricultural University

College of Horticulture，Nanjing Agricultural University

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Effects of nitrogen application rate on agronomic traits of Brassica napus L. and screening of nitrogen-efficient genotypes

DOI：10.13300/j.cnki.hnlkxb.2025.05.006

摘要

Abstract

关键词

Keywords

references