Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS

ZHENG Meijie; ZHENG Tengfei; LIU Chunhua; WANG Mingyue; LI Xiujuan

doi:10.13300/j.cnki.hnlkxb.2025.04.030

您当前的位置：

首页 >

文章列表页 >

Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS

Techniques and methods | 更新时间：2026-11-05

- Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS
- Journal of Huazhong Agricultural University Vol. 44, Issue 4, Pages: 310-317(2025)
- 作者机构：
  
  1.华中农业大学食品科学技术学院，武汉 430070
  2.中国热带农业科学院分析测试中心，海口 570000
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.04.030
  CLC： O657.7
- Received：20 May 2024，
  
  Published：05 July 2025
- 稿件说明：
移动端阅览
郑美杰，郑腾飞，刘春华，等.双棉签棒固相微萃取结合UPLC-MS/MS快速检测香蕉中农药残留［J］.华中农业大学学报，2025，44（4）：310‐317.

ZHENG Meijie,ZHENG Tengfei,LIU Chunhua,et al.Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS[J].Journal of Huazhong Agricultural University,2025,44(04):310-317.
郑美杰，郑腾飞，刘春华，等.双棉签棒固相微萃取结合UPLC-MS/MS快速检测香蕉中农药残留［J］.华中农业大学学报，2025，44（4）：310‐317. DOI： 10.13300/j.cnki.hnlkxb.2025.04.030.

ZHENG Meijie,ZHENG Tengfei,LIU Chunhua,et al.Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS[J].Journal of Huazhong Agricultural University,2025,44(04):310-317. DOI： 10.13300/j.cnki.hnlkxb.2025.04.030.

摘要

为简化香蕉中多农药残留检测步骤，缩短检测时长，降低分析检测成本，采用固相微萃取技术结合超高效液相色谱-串联质谱建立香蕉中11种常见农药残留的快速准确检测方法。以棉签棒作为支撑载体制备2种广谱性大容量萃取头，以双萃取头模式对稀释后的香蕉匀浆样品中农药进行萃取，以微量甲醇解吸萃取头上农药后再利用超高效液相色谱-串联质谱检测，采用基质外标法定量。结果显示，11种农药在香蕉基质中的检出限为0.23~2.43 μg/kg，定量限为0.68~7.36 μg/kg；不同加标水平下回收率为86.2%~109.8%，相对标准偏差为0.6%~11.2%，符合农药残留检测相关规定。研究结果表明，新建方法简单、快速、灵敏、准确、成本低，能满足香蕉中多农药残留的快速检测要求。

Abstract

A rapid and accurate method for detecting 11 common pesticide residues in bananas was established with solid-phase microextraction combined with ultra-high performance liquid chromatography tandem mass spectrometry （SPME-UPLC-MS/MS） to simplify the steps of detecting multi-pesticide residues in bananas， shorten the time of detection， and reduce the cost of detection. Swabs were used as support carriers to prepare the double extraction heads with broad-spectrum and large-capacity. The pesticides were extracted from diluted banana homogenate samples with the double extraction heads. Trace amounts of methanol were used to desorb pesticides from the heads， followed by detection with SPME-UPLC-MS/MS and quantification with method of matrix-matched external standard. The results showed that the limit of detection of 11 pesticides in bananas was 0.23-2.43 μg/kg， with the limit of quantification of 0.68-7.36 μg/kg. The rate of recovery at different spiked levels was 86.2%-109.8%， with a relative standard deviation of 0.6%-11.2%， which complies with the relevant regulations of detecting pesticide residues. It is indicated that the established method is simple， rapid， sensitive， accurate， and cost-low， and can meet the requirements for rapid detection of multi-pesticide residues in bananas.

关键词

Keywords

references

ZAMBONIN C，ARESTA A.Recent applications of solid phase microextraction coupled to liquid chromatography［J/OL］.Separations，2021，8（3）：34［2024-05-20］.https：//doi.org/10.3390/separations8030034.

CHEN J，PAWLISZYN J B.Solid phase microextraction coupled to high-performance liquid chromatography［J］.Analytical chemistry，1995，67（15）：2530-2533.

CHEN Y，SIDISKY L M.Improvement of solid phase microextraction fiber assembly and interface for liquid chromatography［J］.Analytica chimica acta，2012，743：61-68.

ZHANG W P，ZHANG Z X，MENG J W，et al.Adsorptive behavior and solid-phase microextraction of bare stainless steel sample loop in high performance liquid chromatography［J］.Journal of chromatography A，2014，1365：19-28.

BU Y N，FENG J J，TIAN Y，et al.An organically modified silica aerogel for online in-tube solid-phase microextraction［J］.Journal of chromatography A，2017，1517：203-208.

SUN M，BU Y N，XIN X B，et al.Polyurethane functionalized silica aerogel for in-tube solid-phase microextraction of estrogens prior to high performance liquid chromatography detection［J/OL］.Microchemical journal，2022，181：107699［2024-05-20］.https：//doi.org/10.1016/j.microc.2022.107699.

陈艺鸣.固相微萃取在食品安全检验中的应用研究［J］.中国食品工业，2022（16）：79-81.CHEN Y M.Study on application of solid-phase microextraction in food safety inspection［J］.China food industry，2022（16）：79-81 （in Chinese） .

KASPERKIEWICZ A，PAWLISZYN J.Multi-class pesticide analysis in Cannabis oil using coated blade spray and solid-phase microextraction with liquid chromatography coupled to mass spectrometry［J/OL］.Talanta，2021，225：122036［2024-05-20］.https：//doi.org/10.1016/j.talanta.2020.122036.

DINMOHAMMADPOUR Z，YAMINI Y，NAZRAZ M，et al.A new configuration for in-tube solid phase microextraction based on a thin-film coating［J/OL］.Microchemical journal，2021，171：106869［2024-05-20］.https：//doi.org/10.1016/j.microc.2021.106869.

宫小明，李凯，许文娟，等.棉签固相微萃取结合高效液相色谱-串联质谱法检测蜂蜜中9种大环内酯类抗生素［J］.分析试验室，2024，43（4）：535-540.GONG X M，LI K，XU W J，et al.Quantification of macrolide antibiotics in honey by using cotton swab based solid-phase microextraction combined with high performance liquid chromatography-tandem mass spectrometry［J］.Chinese journal of analysis laboratory，2024，43（4）：535-540 （in Chinese with English abstract） .

邹冬梅，范琼.世界香蕉生产、贸易现状与产业展望［J］.广东农业科学，2022，49（7）：131-140.ZOU D M，FAN Q.Present situation of globle banana production and trade and prospect for banana industry［J］.Guangdong agricultural sciences，2022，49（7）：131-140 （in Chinese with English abstract） .

杨凤，刘灿灿，邓贵明，等.香蕉营养品质与功能特性研究进展［J］.广东农业科学，2022，49（10）：146-154.YANG F，LIU C C，DENG G M，et al.Research progress of banana nutritional quality and functional properties［J］.Guangdong agricultural sciences，2022，49（10）：146-154 （in Chinese with English abstract） .

KRITSI E，TSIAKA T，SOTIROUDIS G，et al.Potential health benefits of banana phenolic content during ripening by implementing analytical and in silico techniques［J/OL］.Life，2023，13（2）：332［2024-05-20］.https：//doi.org/10.3390/life13020332.

SARMA P P，GURUMAYUM N，VERMA A K，et al.A pharmacological perspective of banana：implications relating to therapeutic benefits and molecular docking［J］.Food and function，2021，12（11）：4749-4767.

TANG Z H，WEI Y，WANG D，et al.Risk assessment of 369 pesticide residues in banana from Hainan Province of China through UPLC-Q-TOF/MS［J/OL］.Journal of food composition and analysis，2023，123：105638［2024-05-20］.https：//doi.org/10.1016/j.jfca.2023.105638.

马晨，张群，刘春华，等.香蕉果实中杀虫剂的残留分布及膳食风险［J］.江苏农业科学，2022，50（9）：185-190.MA C， ZHANG Q，LIU C H，et al.Residue distribution and dietary risks of pesticides in banana fruit［J］.Jiangsu agricultural sciences，2022，50（9）：185-190 （in Chinese with English abstract） .

马晨，张群，刘春华，等.香蕉果实中杀菌剂的残留、分布与膳食评估［J］.热带作物学报，2022，43（1）：153-164.MA C，ZHANG Q，LIU C H，et al.Residue and distribution of fungicides in banana fruits and dietary risk assessment［J］.Chinese journal of tropical crops，2022，43（1）：153-164 （in Chinese with English abstract） .

闫君，陈婷，张文，等.香蕉中农药残留短期膳食摄入风险与联合暴露风险分析［J］.食品安全导刊，2024（10）：70-76.YAN J，CHEN T，ZHANG W，et al.Analysis of short-term dietary intake risk and combined exposure risk of pesticide residues in bananas［J］.China food safety magazine，2024（10）：70-76 （in Chinese with English abstract） .

CARNEIRO R P，OLIVEIRA F A S，MADUREIRA F D，et al.Development and method validation for determination of 128 pesticides in bananas by modified QuEChERS and UHPLC-MS/MS analysis［J］.Food control，2013，33（2）：413-423.

WANG Y，SONG Q M，WANG F，et al.Bagging and non-bagging treatment on the dissipation and residue of four mixed application pesticides on banana fruit［J］.Journal of the science of food and agriculture，2021，101（8）：3472-3480.

ZHENG K M，WU X P，CHEN J N，et al.Establishment of an LC-MS/MS method for the determination of 45 pesticide residues in fruits and vegetables from Fujian，China［J/OL］.Molecules，2022，27（24）：8674［2024-05-20］.https：//doi.org/10.3390/molecules27248674.

门雪.热带水果中杀虫剂和杀菌剂筛查技术及降解规律研究［D］.泰安：山东农业大学，2023.MEN X.Study on screening technology and degradation law of pesticides and fungicides in tropical fruits［D］.Taian：Shandong Agricultural University，2023 （in Chinese with English abstract） .

HARMOKO H，PUTRA G K，MUNAWAR H，et al.Thermochemical degradation investigation of pesticide residues in banana homogenate［J/OL］.Food control，2023，143：109329［2024-05-20］.https：//doi.org/10.1016/j.foodcont.2022.109329.

DA SILVA SOUSA J，DO NASCIMENTO H O，DE OLIVEIRA GOMES H，et al.Pesticide residues in groundwater and surface water：recent advances in solid-phase extraction and solid-phase microextraction sample preparation methods for multiclass analysis by gas chromatography-mass spectrometry［J/OL］.Microchemical journal，2021，168：106359［2024-05-20］.https：//doi.org/10.1016/j.microc.2021.106359.

吴秋霞，易齐贤，陈曦，等.广热带睡莲的挥发态气味物质检测及优良芳香品种筛选［J］.华中农业大学学报，2024，43（3）：230-239.WU Q X，YI Q X，CHEN X，et al.Detecting volatile aroma substances and screening elite aromatic varieties in pantropical water lilies［J］.Journal of Huazhong Agricultural University，2024，43（3）：230-239 （in Chinese with English abstract） .

LORD H L.Strategies for interfacing solid-phase microextraction with liquid chromatography［J］.Journal of chromatography A，2007，1152（1/2）：2-13.

MURTADA K，NAZDRAJIĆ E，PAWLISZYN J.Performance evaluation of extraction coatings with different sorbent particles and binder composition［J］.Analytical chemistry，2023，95（34）：12745-12753.

SHI Y R，CHEN L Y，HUANG J L，et al.The effect of different binders on the comprehensive performance of solid phase microextraction fiber［J］.Analytica chimica acta，2020，1140：50-59.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of nitrogen fertilization combined with straw returning to field on rhizosphere microorganisms in rice

Development of an indirect ELISA for detecting Photobacterium damselae using polyclonal antibodies against two outer membrane proteins

Progress on technologies of biological treatment and resource recovery for wastewater from farming livestock and poultry

Metabolomic comparison of muscle quality in Chinese hooksnout carp （Opsariichthys bidens） between integrated photovoltaic-fishery and conventional pond aquaculture modes

Inhibitory effect of emodin on recombinant strain rPRRSV-DV-EGFP

Related Author

Li Xiujuan

ZHENG Meijie

WANG Mingyue

MEI Zhinan

HUANG Qiyuan

LIU Sanbo

GONG Dalin

ZHANG Yipeng

Related Institution

College of Plant Science and Technology, Huazhong Agricultural University

China Resources Sanjiu (Huangshi) Pharmaceutical Co.,Ltd

Sinopharm Zhonglian Pharmaceutical Co.,Ltd

College of Food Science and Technology，Huazhong Agricultural University/ Key Laboratory of Environment Correlative Dietology，Ministry of Education/ Hubei Key Laboratory of Fruit & Vegetable Processing & Quality Control

College of Food Science and Technology， Huazhong Agricultural University /Key Laboratory of Environment Correlative Dietology， Ministry of Education /Hubei Province Key Laboratory of Fruit & Vegetable Processing & Quality Control

AI问答

⁰