GWAS-based screening of candidate genes and pathways for anti-diarrhea in suckling piglets

HUANG Rui; CHEN Dong; ZHAO Zhenjian; SHEN Qi; CUI Shengdi; WANG Junge; CHEN Ziyang; WU Pingxian; TANG Guoqing

doi:10.13300/j.cnki.hnlkxb.2025.04.023

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GWAS-based screening of candidate genes and pathways for anti-diarrhea in suckling piglets

Animal science·Veterinary medicine | 更新时间：2026-11-05

- GWAS-based screening of candidate genes and pathways for anti-diarrhea in suckling piglets
- Journal of Huazhong Agricultural University Vol. 44, Issue 4, Pages: 239-247(2025)
- 作者机构：
  
  1.猪禽种业全国重点实验室/四川农业大学动物科技学院/农业农村部畜禽生物组学重点实验室/ 畜禽遗传资源发掘与创新利用四川省重点实验室，成都 611130
  2.国家生猪技术创新中心，重庆 402460
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.04.023
  CLC： S858.28
- Received：10 April 2024，
  
  Published：05 July 2025
- 稿件说明：
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黄睿，陈栋，赵真坚，等.基于GWAS的哺乳仔猪抗腹泻候选基因筛选及通路研究［J］.华中农业大学学报，2025，44（4）：239‐247.

HUANG Rui,CHEN Dong,ZHAO Zhenjian,et al.GWAS-based screening of candidate genes and pathways for anti-diarrhea in suckling piglets[J].Journal of Huazhong Agricultural University,2025,44(04):239-247.
黄睿，陈栋，赵真坚，等.基于GWAS的哺乳仔猪抗腹泻候选基因筛选及通路研究［J］.华中农业大学学报，2025，44（4）：239‐247. DOI： 10.13300/j.cnki.hnlkxb.2025.04.023.

HUANG Rui,CHEN Dong,ZHAO Zhenjian,et al.GWAS-based screening of candidate genes and pathways for anti-diarrhea in suckling piglets[J].Journal of Huazhong Agricultural University,2025,44(04):239-247. DOI： 10.13300/j.cnki.hnlkxb.2025.04.023.

摘要

为研究哺乳仔猪抗腹泻的遗传因素和通路，通过对640头腹泻仔猪进行采样和统计分析，选取其中600个样本，包括大白猪453头、长白猪105头和杜洛克猪42头进行低深度重测序（1×），对测序结果质控后开展全基因组关联分析（genome-wide association study，GWAS），在大白猪和长白猪中分别筛选出42个和107个显著SNPs，这些位点上下游20 kb区域分别涉及32个和82个基因，对这些基因进行GO和KEGG富集分析，最终分别确定了6个（

PLA2G4A、C1RL、PTPN6、C1R、PPP1R12A

和

GRID2

）和8个（

DAPK1、TMC8、ITM2C、CHMP4B、CAST、PDE4D、HSPA4

和

GRID2

）可能与哺乳仔猪腹泻性状相关的重要候选基因，其中

GRID2

在大白猪和长白猪中同时被筛选到。结果表明，仔猪腹泻性状候选基因与细胞凋亡、免疫、细胞屏障和物质的跨膜运输相关功能有关。

Abstract

640 piglets with diarrhea were sampled and statistically analyzed to study the genetic factors and pathways for anti-diarrhea in suckling piglets.600 samples including 453 large white pigs，105 landrace pigs and 42 Duroc pigs were selected for the low depth resequencing （1×）.Genome-wide association study（GWAS） was conducted after the quality control of the results of sequencing.42 and 107 significant SNPs were screened in large white pigs and landrace and pigs，respectively，with 32 and 82 genes involved at the upstream and downstream 20 kb regions of these loci.GO and KEGG enrichment analysis was performed on these genes.The results showe

d that 6 important candidate genes including

PLA2G4A，C1RL，PTPN6，C1R，PPP1R12A，and GRID2

and 8 important candidate genes including

DAPK1，TMC8，ITM2C，CHMP4B，CAST，PDE4D，HSPA4，and GRID2

that may be associated with diarrhea traits in suckling piglets were identified，with

GRID2

being screened simultaneously in both large white pigs and landrace pigs.It is indicated that candidate genes for diarrhea in piglets are associated with functions related to cell apoptosis，immunity，cell barrier，and transmembrane transport of substances.

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references

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