Medication pattern of traditional Chinese medicine for treating piglet diarrhea based on cyberpharmacology

ZHANG Jieying; GAO Jingjie; ZENG Guangmeng; WANG Xiaoxiao; TAO Yanfei; WANG Xu

doi:10.13300/j.cnki.hnlkxb.2025.04.022

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Medication pattern of traditional Chinese medicine for treating piglet diarrhea based on cyberpharmacology

Animal science·Veterinary medicine | 更新时间：2026-11-05

- Medication pattern of traditional Chinese medicine for treating piglet diarrhea based on cyberpharmacology
- Journal of Huazhong Agricultural University Vol. 44, Issue 4, Pages: 229-238(2025)
- 作者机构：
  
  华中农业大学动物科学技术学院、动物医学院/国家兽药残留基准实验室，武汉 430070
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.04.022
  CLC： R259;S854.5
- Received：03 June 2024，
  
  Published：05 July 2025
- 稿件说明：
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张洁莹，高敬杰，曾广萌，等.基于网络药理学的中医治疗仔猪腹泻的用药规律分析［J］.华中农业大学学报，2025，44（4）：229‐238.

ZHANG Jieying,GAO Jingjie,ZENG Guangmeng,et al.Medication pattern of traditional Chinese medicine for treating piglet diarrhea based on cyberpharmacology[J].Journal of Huazhong Agricultural University,2025,44(04):229-238.
张洁莹，高敬杰，曾广萌，等.基于网络药理学的中医治疗仔猪腹泻的用药规律分析［J］.华中农业大学学报，2025，44（4）：229‐238. DOI： 10.13300/j.cnki.hnlkxb.2025.04.022.

ZHANG Jieying,GAO Jingjie,ZENG Guangmeng,et al.Medication pattern of traditional Chinese medicine for treating piglet diarrhea based on cyberpharmacology[J].Journal of Huazhong Agricultural University,2025,44(04):229-238. DOI： 10.13300/j.cnki.hnlkxb.2025.04.022.

摘要

为探究中医治疗仔猪腹泻的用药规律和作用机制，检索并收集中国知网、万方、维普等数据库中与治疗仔猪腹泻相关的中医复方，运用Python和SPSS进行数据挖掘，并结合药频、药性、药味、归经、药对、关联规则等用药规律进行分析。通过网络药理学分析核心药对，借助TCMSP数据库和Swiss ADME平台提取成分和靶点，运用STRING数据库对交集靶点进行蛋白质相互作用分析，使用Cytoscape 3.9.1软件的Network Analyze获取靶点的介数，应用Webgestalt数据库进行靶点的GO和KEGG富集分析。结果显示，中医治疗仔猪腹泻用药频次≥5次的有19味药，其中高频药物有茯苓、黄连、白术、党参、陈皮、黄芪等。药性以温（38.13%），药味以甘（33.21%）、苦（33.03%），归经以脾（22.94%）、胃（18.07%）、肺（13.67%）为主；高频药对有白术、茯苓和党参，频次均在10次以上；在关联规则中，党参、白术和茯苓组合联系紧密，筛选和去重可获得组合涉及22个活性成分，靶点672个，GO分析发现靶点与生物过程相关的有12条，与细胞组分相关的有21条，与分子功能相关的有18条，涉及KEGG通路10条。结果表明，治疗仔猪腹泻多用清热、消食和补益药，核心药对以“党参、白术和茯苓”为代表，其活性成分作用于ALB、AKT1、TP53等炎症相关靶点，参与MAPK、Ras和cAMP等通路发挥抗腹泻作用。

Abstract

The prescriptions of traditional Chinese medicine （TCM） compound related to the treatment of piglet diarrhea were collected from CNKI，WanfangData，VP Database and ancient books，and the medication pattern of medication frequency，medicinal properties，medicinal flavors，categorization，medicinal pairs，and association rules was analyzed with Python and SPSS to mine data to study the medication pattern and mechanism of TCM in the treatment of piglet diarrhea.The core pairs of drugs were analyzed with cyber-pharmacology.The components and targets were extracted with TCMSP database and Swiss ADME platform.The intersection targets were analyzed by protein interaction analysis with STRING database.The mediators of targets were obtained with Network Analyze of Cytoscape 3.9.1 software.The GO and KEGG enrichment of the targets were analyzed with webgestalt database.The results showed that there were 19 medicines with a frequency of ≥5 used in TCM for the treatment of piglet diarrhea，among which the medicine with high-frequency was

Poria cocos

，

Coptis chinensis

，

Atractylodes macrocephala

，

Codonopsis pilosula

，Tangerine peel and

Astragalus membranaceus

. 38.13% medicines are warm medicinal properties.33.21% and 33.03% medicines are mainly sweet and bitter medicinal flavor，with the spleen （22.94%），stomach （18.07%），and lungs （13.67%） being the main meridians.The drug pairs with high-frequency are

Codonopsis pilosulae

，

Atractylodes macrocephala

and

Poria cocos

，with the frequency of more than 10 times，and the combination is closely associated.A combination involving 22 active ingredients and 672 targets was obtained with screening and deduplication.The results of GO analysis showed that 12 targets related to biological processes，21 targets related to cellular components，and 18 targets related to molecular functions，involving 10 KEGG pathways.It is indicated that most of the drugs used in the treatment of

piglet diarrhea are heat-clearing，food-eliminating and tonic drugs，and the core pairs of medicine are represented by

Codonopsis pilosulae

，

Atractylodes macrocephala

and

Poria cocos

，with active ingredients acting on the inflammation-related targets including ALB，AKT1 and TP53，and participating in the pathways of MAPK，Ras and cAMP to exert the antidiarrheal effect.

关键词

Keywords

references

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