Laboratory screening of dimefluthrin-resistant strains of <italic style="font-style: italic">Aedes albopictus</italic> and analysis of hereditary modes of resistance

MA Lulu; JIANG Dingxin

doi:10.13300/j.cnki.hnlkxb.2025.04.017

您当前的位置：

首页 >

文章列表页 >

Laboratory screening of dimefluthrin-resistant strains of Aedes albopictus and analysis of hereditary modes of resistance

Plant protection | 更新时间：2026-11-05

- Laboratory screening of dimefluthrin-resistant strains of Aedes albopictus and analysis of hereditary modes of resistance
- Journal of Huazhong Agricultural University Vol. 44, Issue 4, Pages: 174-180(2025)
- 作者机构：
  
  华南农业大学植物保护学院/绿色农药全国重点实验室/天然农药与化学生物学教育部重点实验室，广州 510642
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.04.017
  CLC： R184.31
- Received：08 March 2024，
  
  Published：05 July 2025
- 稿件说明：
移动端阅览
马露露，江定心.白纹伊蚊四氟甲醚菊酯抗性品系室内筛选及抗性遗传分析［J］.华中农业大学学报，2025，44（4）：174‐180.

MA Lulu,JIANG Dingxin.Laboratory screening of dimefluthrin-resistant strains of Aedes albopictus and analysis of hereditary modes of resistance[J].Journal of Huazhong Agricultural University,2025,44(04):174-180.
马露露，江定心.白纹伊蚊四氟甲醚菊酯抗性品系室内筛选及抗性遗传分析［J］.华中农业大学学报，2025，44（4）：174‐180. DOI： 10.13300/j.cnki.hnlkxb.2025.04.017.

MA Lulu,JIANG Dingxin.Laboratory screening of dimefluthrin-resistant strains of Aedes albopictus and analysis of hereditary modes of resistance[J].Journal of Huazhong Agricultural University,2025,44(04):174-180. DOI： 10.13300/j.cnki.hnlkxb.2025.04.017.

摘要

为明确四氟甲醚菊酯抗性白纹伊蚊（

Aedes albopictus

）品系的抗药性水平，选择四氟甲醚菊酯抗性品系，进行杂交和回交试验，确定四氟甲醚菊酯抗性白纹伊蚊种群的显性度（

）、细胞质影响因子和遗传基因。结果显示，FRS种群的抗性基因频率与抗性品系白纹伊蚊接近、FSR种群的敏感性强；FRS种群和FSR种群之间不交叠，分属2个不同的区间，2个杂交种群之间差异显著；4组回交后代BC1（FSR♀×SS♂）、BC2（FRS♀×SS♂）、BC3（FSR♀×RR♂）以及BC4（FRS♀×RR♂）的实际与期望剂量对数-死亡率（LD-P）曲线之间有差别，实际卡方值（χ

）明显大于期望卡方值。研究结果表明，白纹伊蚊对四氟甲醚菊酯的抗性遗传为多基因控制的母系遗传，这种遗传方式将使白纹伊蚊抗性种群迅速发展。在使用四氟甲醚菊酯防治白纹伊蚊时，为达到好的杀虫效果，且尽量避免害虫产生抗药性，就需要注意可能会带来不利影响的多个因素，如用药剂量、杀虫剂使用方法以及用药周期等，不同蚊虫对不同药剂产生的抗药性的遗传方式可能不同，因此在研制药剂以及用药时也要注意。

Abstract

To clarify the resistance level to dimefluthrin in

Aedes albopictus

and to select a strain that is resistant to dimefluthrin，the mechanisms and genetics of the resistance should be studied. Cross and backcross tests were conducted to caculate the 95% confidence interval for the degree of dominance in the two hybrid populations. Additionally，the actual and expected curves of the four groups of backcross offspring were observed，and the actual and expected chi-square values of the four groups of backcross offspring were calculated to determine the degree of dominance （

），identify cytoplasmic influencing factors，and elucidate the genes associated with the heredity of the dimefluthrin-resistant population of

A. albopictus.

The results indicated that the resistance frequency in the FRS population is close to that of the resistant strain （RR），while the sensitivity of the FSR population is notebly high. The FRS and FSR populations did not overlap，indicating a significant difference between the FRS and FSR hybrids. Furthermore，the actual response of the LD-P line for BC1 （FSR♀×SS♂），BC2 （FRS♀×SS♂），BC3 （FSR♀×RR♂） and BC4 （FRS♀×RR♂） differed from the expected response of the LD-P line，with the actual Chi-square value （χ

） being significa

ntly greater than the expected Chi-square value. Therefore，the inheritance of resistance to dimefluthrin in

A. albopictus

is polygenic and exhibits maternal effect，which can lead to the rapid development of resistant populations. When using dimefluthrin to control

A. albopictus

，it is essential to consider several factors that may negatively impact insecticidal efficacy and contribute to the development of resistance. These factors include dosage，application methods and the timing of insecticide use. Different mosquitoes species may have different hereditary modes of resistance to different insecticides，therefore，it is crucial to monitor the development and application of insecticides carefully.

关键词

Keywords

references

LI Y J，XU J B，ZHONG D B，et al.Evidence for multiple-insecticide resistance in urban Aedes albopictus populations in Southern China［J/OL］.Parasites & vectors，2018，11（1）：4［2024-03-08］. https：//doi.org/10.1186/s13071-017-2581-y.

卢娜，周红宁.我国登革热重要媒介白纹伊蚊抗药性研究进展［J］.热带病与寄生虫学，2022，20（3）：165-169. LU N，ZHOU H N.Research progress on the insecticide resistance in the important vector Aedes albopictus of Dengue fever in China［J］.Journal of tropical diseases and parasitology，2022，20（3）：165-169 （in Chinese with English abstract） .

赵春春，周欣欣，李文玉，等. 2020年中国13省份登革热媒介白纹伊蚊抗药性监测及分析研究［J］.中国媒介生物学及控制杂志，2022，33（1）：30-37. ZHAO C C，ZHOU X X，LI W Y，et al. Insecticide resistance surveillance and characteristic analysisof dengue vector Aedes albopictus in 13 pr ovinces of China in 2020［J］. Chinese journal of vector biology and control，2022，33（1）：30-37 （in Chinese with English abstract） .

姜家良，张朝远，沈建华.淡色库蚊对拟除虫菊酯类农药抗药性研究［J］.农药，1986，25（2）：7-8. JIANG J L，ZHANG C Y，SHEN J H.Study on the resistance of Culex pipiens pallens to pyrethroid pesticides［J］.Agrochemicals，1986，25（2）：7-8 （in Chinese） .

郭凤英，吴厚永，李承毅.白纹伊蚊对高效氯氰菊酯的抗药性及其遗传方式［J］.寄生虫与医学昆虫学报，2001，8（2）：103-108. GUO F Y，WU H Y，LI C Y.Genetic form and resistance of beta-cypermethrin of Aedes albopictus Skuse［J］.Acta parasitologica et medica entomologica sinica，2001，8（2）：103-108 （in Chinese with English abstract） .

刘洪霞，冷培恩，徐劲秋，等.白纹伊蚊对溴氰菊酯的抗性选育及抗性风险评估［J］.中华卫生杀虫药械，2015，21（2）：125-127. LIU H X，LENG P E，XU J Q，et al.Resistance selection of Aedes albopictus to deltamethrin and risk assessment［J］.Chinese journal of hygienic insecticides & equipments，2015，21（2）：125-127 （in Chinese with English abstract） .

何凤侠，刘宝琼，廖如燕，等.广州3个口岸白纹伊蚊对5种常用杀虫剂的抗性调查［J］.中华卫生杀虫药械，2016，22（6）：616-617. HE F X，LIU B Q，LIAO R Y，et al.Resistance of Aedes albopictus to five commonly used insecticides at three ports of Guangzhou［J］.Chinese journal of hygienic insecticides & equipments，2016，22（6）：616-617 （in Chinese with English abstract） .

STONE B F. A formula for determining degree of dominance in cases of monofactorial inheritance of resistance to chemicals［J］. Bulletin of the World Health Organization，1968，38（2）：325.

刘芹轩，武予清.植食叶螨抗药性遗传学研究的进展［J］.遗传，1994，16（5）：45-48. LIU Q X，WU Y Q.Review on inheritance of pesticide resistance in phytophagous spider mites［J］.Hereditas，1994，16（5）：45-48 （in Chinese with English abstract） .

GEORGHIOU G P，GARBER M J.Studies on the inheritance of carbamate-resistance in the housefly （ Musca domestica L.）［J］.Bulletin of the World Health Organization，1965，32（2）：181-196.

SOKAL R R，ROHLF F J.Biometry. 3rd ed［J］.New York： Freeman and Company，1995：150-168.

LI T，LIU N N.Inheritance of permethrin resistance in Culex quinquefasciatus ［J］.Journal of medical entomology，2010，47（6）：1127-1134.

闫华超，高岚.线粒体DNA遗传特性的研究新进展［J］.生物技术，2003，13（6）：63-65. YAN H C，GAO L.Research advance in genetic features of mitochondrial DNA［J］.Biotechnology，2003，13（6）：63-65 （in Chinese） .

SIMON C，FRATI F，BECKENBACH A，et al.Evolution，weighting，and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers［J］.Annals of the entomological society of America，1994，87（6）：651-701.

DOS SANTOS PADUAN K，RIBOLLA P E M.Mitochondrial DNA polymorphism and heteroplasmy in populations of Aedes aegypti in Brazil［J］.Journal of medical entomology，2008，45（1）：59-67.

DE MERIDA A M，PALMIERI M，YURRITA M，et al.Mitochondrial DNA variation among Anopheles albimanus populations［J］.The American journal of tropical medicine and hygiene，1999，61（2）：230-239.

GORROCHOTEGUI-ESCALANTE N，MUNOZ M L，FERNANDEZ-SALAS I，et al.Genetic isolation by distance among Aedes aegypti populations along the northeastern coast of Mexico ［J］.The American journal of tropical medicine and hygiene，2000，62（2）：200-209.

DING Y R，YAN Z T，SI F L，et al.Mitochondrial genes associated with pyrethroid resistance revealed by mitochondrial genome and transcriptome analyses in the malaria vector Anopheles sinensis （Diptera：Culicidae）［J］.Pest management science，2020，76（2）：769-778.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Regulation of microclimate-hormone-growth and development in cigar tobacco under greenhouse planting

Improved YOLOv8s-based model for detecting bud eye in tubers of seed potatoes

Recovery of green aqueous ammonia solution from biogas slurry and its performance of absorbing CO2

Identification of SNPs in smg9 gene of Megalobrama amblycephala and its correlation with resistance to Aeromonas hydrophila infection

Genome⁃wide identification and expression analysis of the CoSAD gene family in Camellia oleifera plants with three levels of ploidy

Related Author

No data

Related Institution

No data

AI问答

⁰

Laboratory screening of dimefluthrin-resistant strains of Aedes albopictus and analysis of hereditary modes of resistance

DOI：10.13300/j.cnki.hnlkxb.2025.04.017

摘要

Abstract

关键词

Keywords

references