Detection and species analysis of RNA mycoviruses in four types of edible mushrooms

WANG Xin; LIU Yingying; XIAO Junbo; LI Huaping; LI Pengfei

doi:10.13300/j.cnki.hnlkxb.2025.04.016

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Detection and species analysis of RNA mycoviruses in four types of edible mushrooms

Plant protection | 更新时间：2026-11-05

- Detection and species analysis of RNA mycoviruses in four types of edible mushrooms
- Journal of Huazhong Agricultural University Vol. 44, Issue 4, Pages: 160-173(2025)
- 作者机构：
  
  绿色农药全国重点实验室/华南农业大学植物保护学院，广州510642
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.04.016
  CLC： S432.4
- Received：25 November 2024，
  
  Published：05 July 2025
- 稿件说明：
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王鑫，刘莹莹，肖俊博，等.4类食用菌中RNA病毒的检测和种类分析［J］.华中农业大学学报，2025，44（4）：160‐173.

WANG Xin,LIU Yingying,XIAO Junbo,et al.Detection and species analysis of RNA mycoviruses in four types of edible mushrooms[J].Journal of Huazhong Agricultural University,2025,44(04):160-173.
王鑫，刘莹莹，肖俊博，等.4类食用菌中RNA病毒的检测和种类分析［J］.华中农业大学学报，2025，44（4）：160‐173. DOI： 10.13300/j.cnki.hnlkxb.2025.04.016.

WANG Xin,LIU Yingying,XIAO Junbo,et al.Detection and species analysis of RNA mycoviruses in four types of edible mushrooms[J].Journal of Huazhong Agricultural University,2025,44(04):160-173. DOI： 10.13300/j.cnki.hnlkxb.2025.04.016.

摘要

为明确生产中不同食用菌中的病毒种类，采用高通量测序技术，对53株侧耳属（

Pleurotus

spp.）和10株灵芝属（

Ganoderma

spp.），以及17株金针菇（

Flammulina filiformis

）和12株鸡腿菇（

Coprinus comatus

）的菌株中的真菌病毒进行检测，对新发现的可能新病毒进行PCR扩增和序列分析，在序列比较的基础上进行系统发育树分析。BLASTx序列比对分析结果显示

，

4类92个食用菌菌株共有51条contigs可注释为41种病毒，RT-PCR扩增和序列分析共鉴定到13种可能的新病毒，其中，侧耳属菌株中发现7种，鸡腿菇中发现3种，金针菇中发现1种，灵芝属菌株中发现2种。系统发育分析结果显示，这些病毒分属于+ssRNA病毒、-ssRNA病毒和dsRNA病毒，分布在8个病毒科，包括

Barnaviridae、Botourmiaviridae、Deltaflexiviridae、Mymonaviridae、Partitiviridae、Phenuiviridae、Phlegiviridae

和1个未分类的

Bunyavirales

病毒。研究结果表明

：

4类食用菌中存在较为丰富的真菌病毒。

Abstract

To clarify the types of viruses present in various edible mushrooms during production， high-throughput sequencing technology was employed to detect fungal viruses in 53 strains of

Pleurotus

spp.， 10 strains of

Ganoderma

spp.， 17 strains of

Flammulina filiformis

， and 12 strains of

Coprinus comatus

.PCR amplification and sequence analysis were performed on newly identified potential vi

ruses， and phylogenetic tree analyses were conducted based on sequence comparisons.BLASTx sequence alignment results indicated that a total of 51 contigs were annotated as 41 viruses detected from 92 strains of four types of edible mushrooms.Thirteen potential novel viruses were identified through RT-PCR amplification and sequence analysis， including seven species found in

Pleurotus

spp.， three species in

Coprinus comatus

， one species in

Flammulina filiformis

， and two species in

Ganoderma

spp..Phylogenetic analyses showed that these viruses belong to +ssRNA viruses， -ssRNA viruses， and dsRNA viruses， distributed across wight viral families：

Barnaviridae

，

Botourmiaviridae

，

Deltaflexiviridae

，

Mymonaviridae

，

Partitiviridae

，

Phenuiviridae， Phlegiviridae

， and an unclassified member of the order

Bunyavirales

.In conclusion， there is a relatively abundant presence of fungal viruses in the four types of edible mushrooms.

关键词

Keywords

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