Analysis of endogenous hormone content in sugarcane leaves infected by <italic style="font-style: italic">Fusarium verticillioides</italic> based on HPLC

YANG Cuifang; GAO Yijing; HUANG Hairong; HUANG Dongmei; YANG Hai; SHEN Yaoyang; LI Xiang; WANG Zeping

doi:10.13300/j.cnki.hnlkxb.2025.04.015

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Analysis of endogenous hormone content in sugarcane leaves infected by Fusarium verticillioides based on HPLC

Plant protection | 更新时间：2026-11-05

- Analysis of endogenous hormone content in sugarcane leaves infected by Fusarium verticillioides based on HPLC
- Journal of Huazhong Agricultural University Vol. 44, Issue 4, Pages: 150-159(2025)
- 作者机构：
  
  1.广西壮族自治区农业科学院/农业农村部广西甘蔗生物技术与遗传改良重点实验室/ 广西甘蔗遗传改良重点实验室，南宁530007
  2.广西壮族自治区亚热带作物研究所/广西亚热带特色水果质量安全控制重点实验室，南宁 530001
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.04.015
  CLC： S435.661
- Received：19 March 2024，
  
  Published：05 July 2025
- 稿件说明：
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杨翠芳，高轶静，黄海荣，等.基于HPLC的轮枝镰刀菌侵染后甘蔗叶片内源激素含量分析［J］.华中农业大学学报，2025，44（4）：150‐159.

YANG Cuifang,GAO Yijing,HUANG Hairong,et al.Analysis of endogenous hormone content in sugarcane leaves infected by Fusarium verticillioides based on HPLC[J].Journal of Huazhong Agricultural University,2025,44(04):150-159.
杨翠芳，高轶静，黄海荣，等.基于HPLC的轮枝镰刀菌侵染后甘蔗叶片内源激素含量分析［J］.华中农业大学学报，2025，44（4）：150‐159. DOI： 10.13300/j.cnki.hnlkxb.2025.04.015.

YANG Cuifang,GAO Yijing,HUANG Hairong,et al.Analysis of endogenous hormone content in sugarcane leaves infected by Fusarium verticillioides based on HPLC[J].Journal of Huazhong Agricultural University,2025,44(04):150-159. DOI： 10.13300/j.cnki.hnlkxb.2025.04.015.

摘要

为明确甘蔗内源激素与梢腐病抗性的关系，以我国甘蔗梢腐病主要致病菌轮枝镰刀菌

Fusarium verticillioides

为病原菌，以高抗梢腐病品种粤糖94-128（YT94-128）和感病品种桂糖37号（GT37）为材料，应用高效液相色谱（HPLC）方法检测病原菌接种处理后不同抗性甘蔗品种叶片中5种内源激素含量，并探究其变化规律。结果显示，HPLC法测定甘蔗叶片中赤霉素（gibberellin，GA）、茉莉酸（jasmonic acid，JA）、柠檬酸（citric acid，CA）、苹果酸（malic acid，MA）、水杨酸（salicylic acid，SA），平均回收率分别达99.61%、99.12%、97.36%、96.74%、99.90%，精密度RSD值范围为0.033%~4.220%，表明该方法快速可靠、分离度高，稳定性与重现性良好。对感病品种GT37和抗病品种 YT94-128进行病原菌接种处理后发现，GA含量在两品种中均随甘蔗生长而降低，且GT37的下降趋势更显著；JA含量先升后降，GT37的下降幅度更大；YT94-128 的 CA 含量持续增加，而 GT37合成 CA能力显著下降；MA 含量在 YT94-128中持续上升并趋于稳定，GT37在接种4 d时出现峰值后下降并稳定；SA含量在两品种中均先升后降，YT94-128的变化趋势更为明显。研究表明，甘蔗感染梢腐病后，内源激素的含量差异及变化幅度可作为反映不同品种抗病能力的重要指标。

Abstract

To clarify the relationship between endogenous hormones and resistance to sugarcane pokkah boeng disease，this study utilized

Fusarium verticillioides，

the primary pathogenic fungus of sugarcane pokkah boeng disease in China，as the pathogen，and employed the highly resistant cultivar YT94-128 （Yuetan 94-128） and the susceptible cultivar GT37 （Guangxi Tang 37） as experimental materials. High-performance liquid chromatography （HPLC） was applied to detect the dynamic changes of five endogenous hormones in sugarcane leaves following pathogen inoculation. The results demonstrated that the HPLC method achieved average recovery rates of 99.61%，99.12%，97

.36%，96.74%，and 99.90% for gibberellin （GA），jasmonic acid （JA），citric acid （CA），malic acid （MA）and salicylic acid （SA） in sugarcane leaves，respectively.The precision，indicated by relative standard deviation （RSD） values，ranged from 0.033% to 4.220%，highlighting the method’s rapid analysis，high resolution，and excellent stability and reproducibility. Post-inoculation analysis revealed distinct hormonal patterns： GA content progressively decreased in both cultivars during sugarcane growth，with a more pronounced reduction in GT37. JA content initially increased before decling，showing a greater reduction amplitude in GT37. CA content continuously increased in YT94-128 but significantly decreased in GT37. MA content steadily rose and stabilized in YT94-128，whereas GT37 exhibited a peak at 4 days post-inoculation，followed by a stabilization. SA content in both cultivars displayed an initial increase followed by a decline，with more marked fluctuations in YT94-128. This study demonstrates that the differential concentrations and dynamic patterns of endogenous hormones following pathogen infection may serve as critical indicators for assessing varietal resistance to sugarcane pokkah boeng disease.

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references

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Analysis of endogenous hormone content in sugarcane leaves infected by Fusarium verticillioides based on HPLC

DOI：10.13300/j.cnki.hnlkxb.2025.04.015

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