Symbiotic nitrogen fixation of <italic style="font-style: italic">atpA</italic> gene in <italic style="font-style: italic">Mesorhizobium huakuii</italic> 7653R

YU Fuyan; ZOU Qian; YAN Chunlan; CHENG Guojun

doi:10.13300/j.cnki.hnlkxb.2025.04.013

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Symbiotic nitrogen fixation of atpA gene in Mesorhizobium huakuii 7653R

Resources and environment | 更新时间：2026-11-05

- Symbiotic nitrogen fixation of atpA gene in Mesorhizobium huakuii 7653R
- Journal of Huazhong Agricultural University Vol. 44, Issue 4, Pages: 133-142(2025)
- 作者机构：
  
  中南民族大学生命科学学院，武汉 430074
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.04.013
  CLC： S154.3
- Received：17 June 2024，
  
  Published：05 July 2025
- 稿件说明：
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余福燕，邹倩，阎春兰，等.华癸中慢生根瘤菌atpA基因在共生固氮中的功能研究［J］.华中农业大学学报，2025，44（4）：133‐142.

YU Fuyan,ZOU Qian,YAN Chunlan,et al.Symbiotic nitrogen fixation of atpA gene in Mesorhizobium huakuii 7653R[J].Journal of Huazhong Agricultural University,2025,44(04):133-142.
余福燕，邹倩，阎春兰，等.华癸中慢生根瘤菌atpA基因在共生固氮中的功能研究［J］.华中农业大学学报，2025，44（4）：133‐142. DOI： 10.13300/j.cnki.hnlkxb.2025.04.013.

YU Fuyan,ZOU Qian,YAN Chunlan,et al.Symbiotic nitrogen fixation of atpA gene in Mesorhizobium huakuii 7653R[J].Journal of Huazhong Agricultural University,2025,44(04):133-142. DOI： 10.13300/j.cnki.hnlkxb.2025.04.013.

摘要

为探究华癸中慢生根瘤菌7653R ABC转运体渗透酶基因

atpA

在根瘤菌与豆科植物共生固氮中的作用，利用单交换插入突变获得

atpA

基因突变体，研究其在自生生长及与紫云英共生条件下的功能。结果显示：

atpA

基因突变会使根瘤菌提前进入稳定期，减弱其生物膜形成能力。接种突变株的植株叶片泛黄、缺氮症状突出，其植株高度、地上部分鲜质量、结瘤数和固氮酶活性分别降低了14.3%、33.3%、31.5%和18.8%。通过蛋白质组学分析，突变株类菌体中共鉴定到78个差异表达蛋白，其中23个表达上调，55个表达下调。在这些差异表达蛋白中，有10个氨基酸转运相关蛋白和12个固氮复合物形成相关蛋白。研究表明，华癸中慢生根瘤菌ABC转运体渗透酶基因

atpA

在根瘤菌生长及与宿主紫云英共生过程中起着至关重要的作用。

Abstract

The role of ABC transporter permease gene

atpA

in the symbiotic nitrogen fixation between

Rhizobium

and legumes in

Mesorhizobium huakuii

7653R was studied. The

atpA

mutant strain HKatpA was constructed through homologous recombination. The functions of the mutant stain in the growth and symbiosis of

Astragalus sinicus

L. was studied. The results showed that the mutation in the

atpA

gene caused rhizobia to early enter a stable period and weaken its ability to form biofilm. The plants inoculated with the mutant strain had yellow leaves， and prominent symptoms of nitrogen deficiency， with their plant height， fresh weight of the above-ground parts， number of nodules， and the activity of nitrogen fixing enzyme decreased by 14.3%， 33.3%， 31.5%， and 18.8%， respectively. 78 differentially expressed proteins were identified with proteomics analysis. Compared wit

h wild-type bacteroids， 23 proteins in

atpA

gene mutant bacteroids were up-regulated and 55 proteins were down-regulated. Among the differentially expressed proteins， 10 proteins were related to amino acid transport， and 12 proteins were related to nitrogen-fixing complex formation in nodules. It is indicated that the ABC transporter permease gene

atpA

Mesorhizobium huakuii

plays an important role in the process of growth and symbiosis with the host plant

Astragalus sinicus

L..

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Symbiotic nitrogen fixation of atpA gene in Mesorhizobium huakuii 7653R

DOI：10.13300/j.cnki.hnlkxb.2025.04.013

摘要

Abstract

关键词

Keywords

references