Effects of black rice anthocyanins on growth，antioxidant capacity，immune enzyme activity，and intestinal health of <italic style="font-style: italic">Procambarus clarkii</italic>

ZHANG Qian; HU Wendi; MO Aijie; YANG Huijun; GU Zemao; YUAN Yongchao

doi:10.13300/j.cnki.hnlkxb.2025.03.015

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Effects of black rice anthocyanins on growth，antioxidant capacity，immune enzyme activity，and intestinal health of Procambarus clarkii

更新时间：2026-11-05

- Effects of black rice anthocyanins on growth，antioxidant capacity，immune enzyme activity，and intestinal health of Procambarus clarkii
- Journal of Huazhong Agricultural University Vol. 44, Issue 3, Pages: 128-135(2025)
- 作者机构：
  
  1.华中农业大学水产学院，武汉430070
  2.华中农业大学双水双绿研究院/长江经济带大宗水生生物产业绿色发展教育部工程研究中心/湖北洪山实验室，武汉430070
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.03.015
  CLC： S966.12
- Received：30 September 2024，
  
  Published：06 May 2025
- 稿件说明：
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张千，户文弟，莫爱杰，等.黑米花青素对克氏原螯虾生长、抗氧化能力、免疫酶活和肠道健康的影响［J］.华中农业大学学报，2025，44（3）：128‐135.

ZHANG Qian,HU Wendi,MO Aijie,et al.Effects of black rice anthocyanins on growth，antioxidant capacity，immune enzyme activity，and intestinal health of Procambarus clarkii[J].Journal of Huazhong Agricultural University,2025,44(03):128-135.
张千，户文弟，莫爱杰，等.黑米花青素对克氏原螯虾生长、抗氧化能力、免疫酶活和肠道健康的影响［J］.华中农业大学学报，2025，44（3）：128‐135. DOI： 10.13300/j.cnki.hnlkxb.2025.03.015.

ZHANG Qian,HU Wendi,MO Aijie,et al.Effects of black rice anthocyanins on growth，antioxidant capacity，immune enzyme activity，and intestinal health of Procambarus clarkii[J].Journal of Huazhong Agricultural University,2025,44(03):128-135. DOI： 10.13300/j.cnki.hnlkxb.2025.03.015.

摘要

为综合评估黑米花青素对克氏原螯虾的适用性，试验选取克氏原螯虾450尾，随机分为5组，在饲粮中分别添加0（control group，CON组）、200 g/kg黑米（black rice 200 g/kg， BR200组）、400 g/kg黑米（black rice 400 g/kg， BR400组）、200 mg/kg黑米花青素提取物（black rice anthocyanin extract 200 mg/kg，AC200组）和400 mg/kg黑米花青素提取物（black rice anthocyanin extract 400 mg/kg，AC400组），试验期8周，探究饲料中黑米花青素的不同添加形式和添加水平对克氏原螯虾生长性能、抗氧化能力、免疫机能和肠道健康的影响。结果显示：（1）BR400组的增重率、特定生长率最高，AC400组次之，均显著高于对照组；（2）处理组试验虾血淋巴、肝胰腺和肠道的抗氧化酶活性均有所升高，AC400组血淋巴和肝胰腺的总抗氧化能力（T-AOC）和超氧化物歧化酶（SOD）活性均最高，与BR400没有显著差异，但BR200组肠道的T-AOC水平和SOD活性最高，且显著高于AC400组和对照组；（3）处理组肝胰腺和肠道的酸性磷酸酶（ACP）和碱性磷酸酶（AKP）的活性均显著高于对照组，BR400组血淋巴的多酚氧化酶（PPO）、ACP和AKP活性均最高；（4）AC400组肠道的淀粉酶活性最高；AC200组胰蛋白酶活性最高，处理组的隐窝深度均显著高于对照组。以上结果表明，饲料中添加黑米或黑米花青素提取物均能够有效提高克氏原螯虾生长、抗氧化、免疫和消化性能，建议添加量为400 g/kg黑米或400 mg/kg的黑米花青素提取物。

Abstract

To comprehensively evaluate the applicability of black rice and black rice anthocyanin extract in the feed of

Procambarus clarkii

，450 juvenile crayfish were randomly allocated into five groups.These groups were fed diets supplemented with 0 （control group，CON），200 g/kg black rice （BR200），400 g/kg black rice （BR400），200 mg/kg anthocyanin extract （AC200），or 400 mg/kg anthocyanin extract （AC400） for 8 weeks.The effects of different forms and supplementation levels of black rice anthocyanins in feed on growth performance，antioxidant capacity，immune response，and intestinal health were investigated.The results revealed that：（1） The BR400 group achieved the highest weight gain rate （WGR） and specific growth rate （SGR），showing no significant difference from AC400，and both were significantly higher than those of the control group.（2） Antioxidant enzyme activities in the hemolymph，hepatopancreas，and intestine were elevated across all treatment groups.AC400 exhibited the highest total antioxidant capacity （T-AOC） and superoxide dismutase （SOD） activity in both the hemolymph and hepatopancreas，with no significant difference observed compared to BR400.Notably，BR200 displayed the highest intestinal T-AOC and SOD activity，significantly surpassing both AC400 and CON groups.（3） Acid phosphatase （ACP） and alkaline phosphatase （AKP） activities in hepatopancreas and intestine were significantly elevated across all treatment groups.BR400 exhibited the highest levels of polyphenol oxidase （PPO），ACP，and AKP activities

in the hemolymph.（4） AC400 demonstrated the highest intestinal amylase activity，while AC200 showed the highest trypsin activity.Additionally，crypt depth in all treatment groups was significantly greater than in CON.These findings indicate that dietary supplementation with either 400 g/kg of black rice or 400 mg/kg of black rice anthocyanin extract effectively enhances growth performance，antioxidant capacity，immune function，and digestive efficiency in

P. clarkii

.Optimal supplementation levels are recommended at 400 g/kg of black rice or 400 mg/kg of black rice anthocyanin extract.

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Effects of black rice anthocyanins on growth，antioxidant capacity，immune enzyme activity，and intestinal health of Procambarus clarkii

DOI：10.13300/j.cnki.hnlkxb.2025.03.015

摘要

Abstract

关键词

Keywords

references