Effects of feeding frequency and feeding level on growth，nutrient composition，and biochemical parameters of <italic style="font-style: italic">Procambarus clarkii</italic>

ZHANG Qian; YANG Weijie; MO Aijie; YANG Huijun; GU Zemao; YUAN Yongchao

doi:10.13300/j.cnki.hnlkxb.2025.03.014

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Effects of feeding frequency and feeding level on growth，nutrient composition，and biochemical parameters of Procambarus clarkii

更新时间：2026-11-05

- Effects of feeding frequency and feeding level on growth，nutrient composition，and biochemical parameters of Procambarus clarkii
- Journal of Huazhong Agricultural University Vol. 44, Issue 3, Pages: 120-127(2025)
- 作者机构：
  
  1.华中农业大学水产学院，武汉430070
  2.华中农业大学双水双绿研究院/长江经济带大宗水生生物产业绿色发展教育部工程研究中心/湖北洪山实验室，武汉430070
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.03.014
  CLC： S966.12
- Received：30 September 2024，
  
  Published：06 May 2025
- 稿件说明：
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张千，杨伟杰，莫爱杰，等.投喂频率和投喂水平对克氏原螯虾机体生长、营养成分和生化指标的影响［J］.华中农业大学学报，2025，44（3）：120‐127.

ZHANG Qian,YANG Weijie,MO Aijie,et al.Effects of feeding frequency and feeding level on growth，nutrient composition，and biochemical parameters of Procambarus clarkii[J].Journal of Huazhong Agricultural University,2025,44(03):120-127.
张千，杨伟杰，莫爱杰，等.投喂频率和投喂水平对克氏原螯虾机体生长、营养成分和生化指标的影响［J］.华中农业大学学报，2025，44（3）：120‐127. DOI： 10.13300/j.cnki.hnlkxb.2025.03.014.

ZHANG Qian,YANG Weijie,MO Aijie,et al.Effects of feeding frequency and feeding level on growth，nutrient composition，and biochemical parameters of Procambarus clarkii[J].Journal of Huazhong Agricultural University,2025,44(03):120-127. DOI： 10.13300/j.cnki.hnlkxb.2025.03.014.

摘要

为优化投喂策略，提高饲料利用率、减少环境废物排放，试验选取体质量（4.97±0.11） g的克氏原螯虾810尾采用3×3双因子设计，日投喂频率1 次（F1）、2 次（F2）和3 次（F3），投喂水平为试验虾质量的3%（L3）、4.5%（L4.5）和6%（L6），饲养56 d，探究投喂频率与投喂水平对克氏原螯虾机体的影响。结果显示：生长方面，L4.5和L6试验虾增重率、特定生长率显著高于L3，L6试验虾的增重率、特定生长率和饲料系数显著高于L3、L4；全虾和肌肉常规营养成分方面，L4.5和L6试验虾粗蛋白含量显著高于L3，L6试验虾全虾的粗脂肪含量显著高于L4.5和L3试验组；血清生化指标方面，L6和L4.5试验虾血清的碱性磷酸酶（AKP）、酸性磷酸酶（ACP）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性及丙二醛（MDA）含量显著高于L3组，F1试验虾的MDA含量显著低于F2、F3，2个试验因素有显著的交互作用；结合增重率、存活率、饲料成本计算养殖效益，L4.5试验组的养殖效益显著高于其他试验组，为23.45元/kg左右，L6其次，L3效益最低（13.65元/kg左右），L4.5F2组饲料成本最低，养殖效益最高。综合考虑试验虾生长、健康程度及养殖效益，建议克氏原螯虾稻田养殖过程中以4.5%的投喂水平、投喂频率2次/d为宜。

Abstract

This study aimed to investigate the effects of feeding frequency and feeding levels on the physiological responses of

Procambarus clarkii

，with the goal of optimizing feeding strategies to enhance feed utilization and reduce environmental waste discharge.A total of 810 crayfish，with an average body weight of （4.97±0.11） g were randomly divided into 9 treatment groups，each with 3 replicates，following a 3×3 two-factor experimental design.The daily feeding frequencies were once （F1），twice （F2），and three times （F3），while the feeding levels were set at 3% （L3），4.5% （L4.5），and 6% （L6） of the experimental shrimp’s body weight.The experiment lasted for 56 days.The results showed that the weight gain rate （WGR） and specific growth rate （SGR） of shrimp in the L4.5 and L6 groups were significantly higher than those in the L3 group （

0.05）.The L6 group exhibited significantly higher WGR，SGR，and feed conversion ratio （FCR） compared to the L3 and L4.5 groups （

0.05）.In terms of whole-body and muscle nutrient composition，the crude protein content in the L4.5 and L6 groups was significantly higher than that in the L3 group （

0.05），while the crude fat content in the whole body of the L6 group was significantly higher than in that in the L4.5 and L3 groups.Serum biochemical analysis revealed that levels of alkaline phosphatase （AKP），acid phosphatase （ACP），superoxide dismutase （SOD），catalase （CAT），and malondialdehyde （MDA） in the L6 and L4.5 groups were significantly higher than those in the L3 group （

0.05）.The MDA level in the F1 group was significantly lower than in the F2 and F3 g

roups （

0.05），with significant interaction effects observed between feeding frequency and level （

0.05）.Based on WGR，survival rate，and feed cost，the L4.5 group achieved the highest economic benefit （approximately 23.45 yuan/kg），followed by the L6 group，while the L3 group yielded the lowest profit （13.65 yuan/kg）.The L4.5F2 group exhibited the lowest feed cost and the highest economic return.Considering growth performance，health status，and cost-effectiveness，a feeding level of 4.5% of body weight，administered twice daily，is recommended for rice-crayfish integrated farming systems.

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呼光富，刘香江.克氏原鳌虾生物学特性及其对我国淡水养殖业产生的影响［J］.北京水产，2008（1）：49-51.HU G F，LIU X J.Biological characteristics of Procambarus clarkii and its influence on freshwater aquaculture in China［J］.Journal of Beijing fisheries，2008（1）：49-51 （in Chinese） .

YUE G H，LI J，BAI Z，et al.Genetic diversity and population structure of the invasive alien red swamp crayfish ［J］.Biological invasions，2010，12（8）：2697-2706.

封功能，王爱民，邵荣，等.克氏原螯虾不同生长阶段营养成分分析与评价［J］.江苏农业科学，2011，39（4）：383-385.FENG G N，WANG A M，SHAO R，et al.Analysis and evaluation of nutritional components of Procambarus clarkii at different growth stages［J］.Jiangsu agricultural sciences，2011，39（4）：383-385 （in Chinese） .

SAPKOTA A，SAPKOTA A R，KUCHARSKI M，et al.Aquaculture practices and potential human health risks：current knowledge and future priorities［J］.Environment international，2008，34（8）：1215-1226.

胡倩，王齐帅，黄瑾，等.基于形态性状和SSR标记的克氏原螯虾养殖群体遗传多样性分析［J］.华中农业大学学报，2024，43（3）：258-266.HU Q，WANG Q S，HUANG J，et al.Genetic diversity analysis o f farming Procambarus clarkii population based on morphology traits and SSR markers［J］.Journal of Huazhong Agricultural University，2024，43（3）：258-266 （in Chinese with English abstract） .

王慧.淮安市克氏原螯虾的养殖现状与可持续发展研究［D］.南京：南京农业大学，2013.WANG H.Research on the current situation and sustainable development of Procambarus clarkii aquaculture in Huai'an City［D］.Nanjing：Nanjing Agricultural University，2013 （in Chinese with English abstract） .

董家莲.小龙虾养殖中的技术细节与误区［J］.农业与技术，2019，39（4）：135-136.DONG J L.Technical details and misunderstandings in crayfish culture［J］.Agriculture and technology，2019，39（4）：135-136 （in Chinese） .

陈松文，江洋，汪金平，等.湖北省稻虾模式发展现状与对策分析［J］.华中农业大学学报，2020，39（2）：1-7.CHEN S W，JIANG Y，WANG J P，et al.Situation and countermeasures of integrated rice-crayfish farming in Hubei Province［J］.Journal of Huazhong Agricultural University，2020，39（2）：1-7 （in Chinese with English abstract） .

ZHENG K K，LIANG M Q，FANG W，et al.Effects of feeding frequency and ration level on growth performance and non-specific immunity of juvenile turbot （ Scophthalmus maximus ） at different growth stages［J］.Israeli journal of aquaculture - bamidgeh，2017，1416：1-7.

覃希.投喂频率和投喂水平对吉富罗非鱼幼鱼生长性能和生理机能的影响［D］.南宁：广西大学，2014.QIN X.Effects of feeding frequency and feeding level on growth performance and physiological function of juvenile Gi fu tilapia［D］.Nanning：Guangxi University，2014 （in Chinese with English abstract） .

王建学.红鳍东方鲀幼鱼蛋能比、投喂策略及主要蛋白源消化率研究［D］.上海：上海海洋大学，2020.WANG J X.The study on optimum protein to energy ratio in diet，feeding strategies and apparent digestibility coefficients of selected feed ingredients of juvenile tiger puffer （ Takifugu rubripes ）［D］.Shanghai：Shanghai Ocean University，2020 （in Chinese with English abstract） .

孙晓锋，冯健，陈江虹，等.投喂频率对尼罗系吉富罗非鱼幼鱼胃排空、生长性能和体组成的影响［J］.水产学报，2011，35（11）：1677-1683.SUN X F，FENG J，CHEN J H，et al.Effects of feeding frequency on gastric evacuation，growth benefit and body composition of juvenile genetic improved farmed tilapia strain of Nile tilapia （ Oreochromis niloticus ）［J］.Journal of fisheries of China，2011，35（11）：1677-1683 （in Chinese with English abstract） .

徐革锋，刘洋，李永发，等.不同投喂率对细鳞鲑（ Brachymystax lenok ）幼鱼生长及体成分的影响［J］.海洋与湖沼，2013，44（2）：433-437.XU G F，LIU Y，LI Y F，et al.Effect of feeding rate on growth and body composition of juvenile Brachymystax lenok ［J］.Oceanologia et limnologia sinica，2013，44（2）：433-437 （in Chinese with English abstract） .

MIZANUR R M，PARK G，YUN H，et al.The effects of feeding rates in juvenile Korean rockfish （ Sebastes schlegeli ） reared at 17 ℃ and 20 ℃ water temperatures［J］.Aquaculture international，2014，22（3）：1121-1130.

EROLDOĞAN O T，KUMLU M，AKTAŞ M.Optimum feeding rates for European sea bass Dicentrarchus labrax L.reared in seawater and freshwater［J］.Aquaculture，2004，231（1/2/3/4）：501-515.

田红艳.光照与投喂策略对团头鲂幼鱼摄食、生长及免疫的影响研究［D］.南京：南京农业大学，2018.TIAN H Y.Effects of light and feeding strategies on feeding，growth and immunity of juvenile Megalobrama amblycephala ［D］.Nanjing：Nanjing Agricultural University，2018 （in Chinese with English abstract） .

褚志鹏，金佳利，陈细华，等.不同投喂率和投喂频率对杂交鲟幼鱼生长、体成分和生化指标的影响［J］.中国水产科学，2020，27（2）：177-185.CHU Z P，JIN J L，CHEN X H，et al.Effects of different feeding rates and frequencies on the growth performance，body composition，and biochemical parameters of juvenile hybrid sturgeon［J］.Journal of fishery sciences of China，2020，27（2）：177-185 （in Chinese with English abstract） .

彭迪，陈效儒，文华，等。饲料脂肪水平对克氏原螯虾亲虾生长性能、肌肉成分、繁殖性能以及血淋巴生化指标的影响［J］.水产学报，2019，43 （10）：2175-2185. PENG D，CHEN X R，WEN H，et al.Effects of dietary lipid levels on growth performance，muscle composition，reproductive performance and he molymph biochemical indices of Procambarus clarkii brood-stock ［J］.Journal of fisheries of China，2019，43（10）：2175-2185 （in Chinese with English abstract） .

SOUSA R M R，AGOSTINHO C A，OLIVEIRA F A，et al.Productive performance of Nile tilapia （ Oreochromis niloticus ） fed at different frequencies and periods with automatic dispenser［J］.Arq Bras Med Vet Zoo，2012，64（1）：192-197.

刘变枝，朱晓鸣，韩冬，等.投喂水平对长吻鮠仔稚鱼生长和存活的影响［J］.水生生物学报，2013，37（2）：261-268.LIU B Z，ZHU X M，HAN D，et al.Effects of feeding level on growth performance of Chinese longsnout catfish （ Leiocassis longirostris Günther） larvae［J］.Acta hydrobiologica sinica，2013，37（2）：261-268 （in Chinese with English abstract） .

袁勇超.胭脂鱼适宜蛋白能量水平、投喂水平和磷需要量及对植物蛋白源的利用研究［D］.武汉：华中农业大学，2011.YUAN Y C.Optimum protein and energy levels，feeding levels and phosphorus requirement and utilization of plant protein sources for Myxocyprinus asiaticus ［D］.Wuhan：Huazhong Agricultural University，2011 （in Chinese with English abstract） .

KARADAL O，TRKMEN G，GROY D.Effect of feeding frequency on growth performance，reproduction efficiency and skin coloration of rusty cichlids （ Iodotropheus sprengerae ）［J］.Journal of applied aquaculture，2020，32（2）：124-138.

TIAN H Y，YANG C C，YU Y B，et al.Dietary cholesterol level affects growth，molting performance and ecdysteroid signal transduction in Procambarus clarkii ［J/OL］.Aquaculture，2020，523：735198［2024-09-30］.https：//doi.org/10.1016/j.aquaculture.2020.735198.

MUTA T，IWANAGA S.The role of hemolymph coagulation in innate immunity［J］.Current opinion in immunology，1996，8（1）：41-47.

LIU X L，XI Q Y，Y ANG L，et al.The effect of dietary Panax ginseng polysaccharide extract on the immune responses in white shrimp， Litopenaeus vannamei ［J］.Fish & shellfish immunology，2011，30（2）：495-500.

何海琪，孙凤.中国对虾酸性和碱性磷酸酶的特性研究［J］.海洋与湖沼，1992，23（5）：555-560.HE H Q，SUN F.Studies on the characteristics of acid and alkaline phosphatases in Chinese shrimp， Penaeus chinensis ［J］.Oceanologia et limnologia sinica，1992，23（5）：555-560 （in Chinese with English abstract） .

王艺超，梁萌青，郑珂珂.不同投喂水平对刺参（ Apostichopus japonicus Selenka）生长、消化酶及免疫相关酶活性的影响［J］.渔业科学进展，2016，37（1）：87-92.WANG Y C，LIANG M Q，ZHENG K K.Effects of different ration levels on growth，activities of digestive enzymes and immunity-related enzymes in Apostichopus japonicus Selenka［J］.Progress in fishery sciences，2016，37（1）：87-92 （in Chinese with English abstract） .

GUO K，RUAN G L，FAN W H，et al.The effect of nitrite and sulfide on the antioxidant capacity and microbial composition of the intestines of red swamp crayfish， Procambarus clarkii ［J］.Fish & shellfish immunology，2020，96：290-296.

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Effects of feeding frequency and feeding level on growth，nutrient composition，and biochemical parameters of Procambarus clarkii

DOI：10.13300/j.cnki.hnlkxb.2025.03.014

摘要

Abstract

关键词

Keywords

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