Effects of crayfish on microbial diversity and carbon sequestration in paddy soil

ZHANG Wanyang; MA Tianqiao; WANG Jinping; GU Zemao; LI Xiaokun

doi:10.13300/j.cnki.hnlkxb.2025.03.009

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Effects of crayfish on microbial diversity and carbon sequestration in paddy soil

更新时间：2026-11-05

- Effects of crayfish on microbial diversity and carbon sequestration in paddy soil
- Journal of Huazhong Agricultural University Vol. 44, Issue 3, Pages: 74-83(2025)
- 作者机构：
  
  1.华中农业大学双水双绿研究院，武汉，430070
  2.华中农业大学资源与环境学院，武汉 430070
  3.华中农业大学植物科学技术学院，武汉 430070
  4.华中农业大学水产学院，武汉 430070
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.03.009
  CLC： S511;S966.12
- Received：02 September 2024，
  
  Published：06 May 2025
- 稿件说明：
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张万洋，马天桥，汪金平，等.克氏原螯虾对稻田土壤微生物多样性和碳固存的影响［J］.华中农业大学学报，2025，44（3）：74‐83.

ZHANG Wanyang,MA Tianqiao,WANG Jinping,et al.Effects of crayfish on microbial diversity and carbon sequestration in paddy soil[J].Journal of Huazhong Agricultural University,2025,44(03):74-83.
张万洋，马天桥，汪金平，等.克氏原螯虾对稻田土壤微生物多样性和碳固存的影响［J］.华中农业大学学报，2025，44（3）：74‐83. DOI： 10.13300/j.cnki.hnlkxb.2025.03.009.

ZHANG Wanyang,MA Tianqiao,WANG Jinping,et al.Effects of crayfish on microbial diversity and carbon sequestration in paddy soil[J].Journal of Huazhong Agricultural University,2025,44(03):74-83. DOI： 10.13300/j.cnki.hnlkxb.2025.03.009.

摘要

为探究稻-虾种养模式不同耕层中稻田土壤微生物多样性及碳固存的差异，采用水稻单作和稻-虾种养模式田间对比试验，研究不同水稻种植模式对耕层（0~20 cm）和亚耕层（20~40 cm）土壤微生物群落多样性和稳定性以及有机碳组分的影响。结果显示，与水稻单作模式相比，稻-虾种养模式耕层和亚耕层土壤有机碳含量分别提高了7.3%和35.8%。亚耕层土壤真菌群落多样性明显下降，Chao1指数、丰富度指数和ACE指数分别降低了6.9%、7.2%和8.7%；而对耕层土壤微生物群落多样性无显著影响。稻-虾种养模式显著提高了耕层和亚耕层土壤细菌和真菌共现性网络的网络边数、连接度和平均度，从而提高了其网络稳定性。可见，稻-虾综合种养模式可显著提高亚耕层土壤有机碳积累和微生物群落稳定性，促进了稻田土壤生物肥力的提升。

Abstract

A field-based comparative experiment between rice monoculture （RM） and rice-crayfishfarming models（RC） was conducted to study the effects of introducing crayfish （

Procambarus clarkii

） into paddy fields on the microbial diversity and the carbon sequestration in soil.The impacts of different rice cultivation models on the diversity and stability of microbial community，and the fractions of organic carbon in the topsoil （0-20 cm） and subsoil （20-40 cm） were investigated.The results showed that the content of organic carbon in topsoil under the RC was 7.3% higher than that under RM，with a significant increase of 35.8% in the content of organic carbon in subsoil.The diversity of fungal community in the subsoil significantly decreased，with a decrease in the index of Chao1，Richness，and ACE indexes of 6.9%，7.2%，and 8.7%，respectively，while there was no significant effect on the diversity of microbial community in the topsoil.The RC significantly enhanced the network size，connectivity，and average degree of co-occurrence networks for bacteria and fungi in both topsoil and subsoil，thereby improving the stability of network.It is indicated that the RC significantly increases the accumulation of organic carbon and the stability of microbial community in subsoil，promoting the biological fertility in paddy soil.

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references

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