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Factors affecting homoacetogenesis of volatile fatty acids with CO2
Food science and agricultural engineering | 更新时间：2026-11-05
|
Factors affecting homoacetogenesis of volatile fatty acids with CO2
- Journal of Huazhong Agricultural University Vol. 44, Issue 2, Pages: 293-300(2025)
- 作者机构：
华中农业大学工学院，武汉 430070
- 作者简介：
- 基金信息：
DOI：10.13300/j.cnki.hnlkxb.2025.02.030
CLC： Q939.9;S216.3Received：13 March 2024，
Published：06 March 2025
- 稿件说明：
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申瑞延，姚冬杰，陈望学，等.利用CO2合成挥发性脂肪酸的同型产乙酸反应的影响因素［J］.华中农业大学学报，2025，44（2）：293‐300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030.
SHEN Ruiyan,YAO Dongjie,CHEN Wangxue,et al.Factors affecting homoacetogenesis of volatile fatty acids with CO2[J].Journal of Huazhong Agricultural University,2025,44(02):293-300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030.
申瑞延，姚冬杰，陈望学，等.利用CO2合成挥发性脂肪酸的同型产乙酸反应的影响因素［J］.华中农业大学学报，2025，44（2）：293‐300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030. DOI：
SHEN Ruiyan,YAO Dongjie,CHEN Wangxue,et al.Factors affecting homoacetogenesis of volatile fatty acids with CO2[J].Journal of Huazhong Agricultural University,2025,44(02):293-300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030. DOI：
Factors affecting homoacetogenesis of volatile fatty acids with CO2
摘要
为了能够以低成本的方式实现CO
2
的资源化利用，同时获得具有高价值的挥发性脂肪酸（volatile fatty acids，VFAs），利用厌氧污泥进行同型产乙酸菌的富集培养，并分别以葡萄糖、CO
2
和H
2
为底物，利用同型产乙酸菌的自养特性，探讨将CO
2
进行资源转化的能力以及顶空H
2
/CO
2
比例、碳源种类及pH值对同型产乙酸反应产物合成的影响。结果显示，高pH值有利于同型产乙酸菌利用H
2
/CO
2
混合气体进行自养反应，当pH=5、
<math id="M1"><msub><mrow><mi>V</mi></mrow><mrow><msub><mrow><mi mathvariant="normal">H</mi></mrow><mrow><mn mathvariant="normal">2</mn></mrow></msub></mrow></msub><mo>∶</mo><msub><mrow><mi>V</mi></mrow><mrow><mi mathvariant="normal">C</mi><msub><mrow><mi mathvariant="normal">O</mi></mrow><mrow><mn mathvariant="normal">2</mn></mrow></msub></mrow></msub></math>
https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=76661440&type=
3.13266683
https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=76661473&type=
14.22399998
=3∶1时的VFAs（包括乙酸、丙酸和丁酸）产量最高，达到3 369.89 mg/L，乙酸占比为76.1%。当以葡萄糖与H
2
/CO
2
混合气体作为共同碳源时，同型产乙酸菌会优先利用葡萄糖进行异养反应，随后开始利用H
2
/CO
2
混合气体。相关性分析表明，VFAs含量与pH值之间呈弱正相关，而与H
2
/CO
2
呈现出负相关趋势，表明CO
2
含量对同型产乙酸菌的自养途径影响更大。此外，建立的同型产乙酸反应的BP神经网络预测模型，可以准确预测以H
2
/CO
2
混合气体为碳源时同型产乙酸菌合成的VFAs产量（
R
2
=0.966）。
Abstract
Industrial syngas contains a large amount of CO
2
， and the utilization of CO
2
resources is of great significance to the current improvement of environment. The homoacetogenesis in the process of anaerobic fermentation has characteristics of autotrophy and heterotrophy. It can use CO
2
and H
2
to produce acetic acid， and degrade glucose to produce volatile fatty acids （VFAs）. The autotrophic characteristics of homoacetogenic bacteria were used to study its ability to convert CO
2
into resources and the effects of headspace H
2
/CO
2
ratio， the type of carbon sources and pH on the synthesis of homoacetogenic reaction products. The results showed that high pH was beneficial for homoacetogenic bacteria to utilize H
2
/CO
2
mixed gas for autotrophic reactions. When pH=5 and H
2
/CO
2
=3∶1， the yield of VFAs including acetic acid， propionic acid and butyric acid was the highest， reaching 3 369.89 mg/L， and the proportion of acetic acid was 76.1%. When glucose and H
2
/CO
2
mixed gas were used as a common carbon source， the homoacetogenic bacteria preferentially used glucose for heterotrophic reaction， and then the H
2
/CO
2
mixed gas began to be utilized. The results of correlation analysis showed that there was a weak positive correlation between the content of VFAs an
d the value of pH， but a negative correlation with H
2
/CO
2
， indicating that the content of CO
2
has a greater impact on the autotrophic pathway of homoacetogens. A prediction model with BP neural network for homoacetogenic reaction was established， which accurately predicted the yield of VFAs synthesized by homoacetogenic bacteria with H
2
/CO
2
mixed gas as the carbon source（
R
2
=0.966）.
关键词
Keywords
references
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