Effects of fertilization treatments on microbial community in dryland red soils revealed by experiments of long-term positioning-based metagenomics

YU Teng; LI Shunjiang; WAN Ziwei; QUE Zhengyang; HUANG Guoqin; XU Huifang

doi:10.13300/j.cnki.hnlkxb.2025.01.016

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Effects of fertilization treatments on microbial community in dryland red soils revealed by experiments of long-term positioning-based metagenomics

Resources and environment | 更新时间：2026-11-05

- Effects of fertilization treatments on microbial community in dryland red soils revealed by experiments of long-term positioning-based metagenomics
- Journal of Huazhong Agricultural University Vol. 44, Issue 1, Pages: 148-155(2025)
- 作者机构：
  
  江西农业大学生态科学研究中心，南昌330045
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.01.016
  CLC： S154.3
- Received：06 May 2024，
  
  Published：15 January 2025
- 稿件说明：
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余腾，李顺江，万子维，等.基于长期定位试验的不同施肥处理下旱地红壤微生物的宏基因组学分析［J］.华中农业大学学报，2025，44（1）：148‐155.

YU Teng,LI Shunjiang,WAN Ziwei,et al.Effects of fertilization treatments on microbial community in dryland red soils revealed by experiments of long-term positioning-based metagenomics[J].Journal of Huazhong Agricultural University,2025,44(01):148-155.
余腾，李顺江，万子维，等.基于长期定位试验的不同施肥处理下旱地红壤微生物的宏基因组学分析［J］.华中农业大学学报，2025，44（1）：148‐155. DOI： 10.13300/j.cnki.hnlkxb.2025.01.016.

YU Teng,LI Shunjiang,WAN Ziwei,et al.Effects of fertilization treatments on microbial community in dryland red soils revealed by experiments of long-term positioning-based metagenomics[J].Journal of Huazhong Agricultural University,2025,44(01):148-155. DOI： 10.13300/j.cnki.hnlkxb.2025.01.016.

摘要

为探究旱地红壤微生物群落与不同施肥制度之间的关系，采用宏基因组测序，比较分析长期定位试验地4种施肥处理：不施肥（CK）、施氮磷钾肥（NPK）、施有机肥（OM）、氮磷钾肥和有机肥混合施用（NPKOM）的土壤微生物群落组成及多样性。结果显示：相比对照，施用有机肥和氮磷钾肥和有机肥混合施用显著增加放线菌门（Actinobacteria）和变形菌门（Proteobacteria）的丰度，降低了绿弯菌门（Chloroflexi）的丰度；施肥显著改变微生物群落结构，施用无机肥降低土壤微生物数量，施用有机肥和无机有机混合施用则增加土壤微生物数量；土壤微生物群落在门水平与pH、全磷（TP）、土壤有机质（SOM）、碱解氮（AN）和有效磷（AP ）含量显著相关，pH是影响土壤微生物群落结构的关键因素；不同施肥处理下微生物代谢功能差异显著。

Abstract

Sequencing of the metagenome was used to comparatively analyze the composition and the diversity of microbial communities in soil under the four fertilizations including no fertilization （CK）， nitrogen， phosphorus and potassium fertilizers （NPK）， organic fertilizer （OM）， and organic fertilizer mixed with nitrogen， phosphorus and potassium fertilizers （NPKOM） in experiments of long-term positioning to study the relationship between the microbial communities in dryland red soils and the systems of fertilization. The results showed that OM and NPKOM significantly increased the abundance of Actinobacteria and Proteobacteria compared to CK， but decreased the abundance of Chloroflexi. Fertilization significantly changed the structure of microbial community in soil， with OM reducing and NPKOM increasing the number of microorganisms in soil. The microbial community in soil was significantly correlated with pH and the content of total phosphorus （TP）， soil organic matter （SOM）， alkali-hydrolyzable nitrogen （AN）， and available phosphorus （AP） at the level of phylum. pH was a key factor affecting the structure of microbial communities in soil. There were significant differences in metabolic functions of microorganisms under different fertilizations. It will provide a certain theoretical basis for the rational fertilization in dryland red soils in China.

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