Effects of mineralization and deposition of nano silicon under low pH on aluminum tolerance in root tip and root border cells of pea

TANG Jiao; FENG Yingming; CHEN Xingyun; GUO Yiwei; ZHENG Yuxin; TIAN Xiao; LI Xuewen; YU Min

doi:10.13300/j.cnki.hnlkxb.2025.01.015

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Effects of mineralization and deposition of nano silicon under low pH on aluminum tolerance in root tip and root border cells of pea

Resources and environment | 更新时间：2026-11-05

- Effects of mineralization and deposition of nano silicon under low pH on aluminum tolerance in root tip and root border cells of pea
- Journal of Huazhong Agricultural University Vol. 44, Issue 1, Pages: 137-147(2025)
- 作者机构：
  
  佛山大学国际膜生物学与环境研究中心/农业与生物工程学院，佛山 528200
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.01.015
  CLC： S311
- Received：19 January 2024，
  
  Published：15 January 2025
- 稿件说明：
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唐娇，冯英明，陈醒韵，等.低pH条件下纳米硅矿化沉积对豌豆根尖和根边缘细胞耐铝性的影响［J］.华中农业大学学报，2025，44（1）：137‐147.

TANG Jiao,FENG Yingming,CHEN Xingyun,et al.Effects of mineralization and deposition of nano silicon under low pH on aluminum tolerance in root tip and root border cells of pea[J].Journal of Huazhong Agricultural University,2025,44(01):137-147.
唐娇，冯英明，陈醒韵，等.低pH条件下纳米硅矿化沉积对豌豆根尖和根边缘细胞耐铝性的影响［J］.华中农业大学学报，2025，44（1）：137‐147. DOI： 10.13300/j.cnki.hnlkxb.2025.01.015.

TANG Jiao,FENG Yingming,CHEN Xingyun,et al.Effects of mineralization and deposition of nano silicon under low pH on aluminum tolerance in root tip and root border cells of pea[J].Journal of Huazhong Agricultural University,2025,44(01):137-147. DOI： 10.13300/j.cnki.hnlkxb.2025.01.015.

摘要

为探究不同pH铝环境条件下纳米硅矿化对豌豆根尖及根边缘细胞保护机制的影响，通过聚乙烯亚胺（polyethyleneimine， PEI）诱导硅静电吸附形成纳米硅壳进行仿生矿化的方式，解析不同pH（pH 3.5、4.5、5.5）条件下纳米硅对豌豆根尖及根边缘细胞缓解铝毒的生理调控机制。结果显示，各pH的铝处理都降低了根边缘细胞活性，而加硅处理后细胞应对铝毒环境的细胞活性显著提高，加硅处理细胞内的铝含量和活性氧（reactive oxygen species，ROS）含量低于缺硅处理且加硅后细胞线粒体膜电位上升；铝毒抑制了豌豆根尖伸长，在pH 3.5铝处理条件下加硅缓解根尖抑制作用的效果不明显，但pH 4.5、5.5条件下根尖伸长量显著增加；随着铝处理pH值升高，根尖铝含量降低，且加硅处理后根尖的铝含量都高于缺硅处理；硅使得细胞壁上更多带负电的官能团参与抗铝胁迫，降低了细胞壁各组分（包括果胶、纤维素、半纤维素、蛋白质等）的吸收峰值；pH 4.5和5.5铝环境条件下细胞表面形成纳米硅壳，硅铝在细胞壁上沉积形成铝硅酸盐。结果表明，低pH条件下纳米硅在细胞上的矿化沉积能够缓解铝离子毒害作用。

Abstract

The mechanism of nano silicon physiologically regulating the alleviation of aluminum toxicity in the root tip and root edge cells of pea under low pH including pH 3.5， pH 4.5， and pH 5.5 was analyzed by PEI–induced silica electrostatic adsorption to form nano-silica shells for biomimetic mineralization to study the effects of the mineralization of nano silicon on the protective mechanism of the root tip and root border cells of pea under different pH and aluminum environments. The results showed that the treatment of Aluminum at various levels of pH decreased the activity of root border cells， while the treatment of silicon significantly increased the activity of cell in response to aluminum toxicity. The content of aluminum and ROS in silicon treated cells were lower than that in silicon deficient cells， and the mitochondrial membrane potential of the cells increased after the addition of silicon. Aluminum toxicity inhibited the elongation of root tip in peas. The effect of adding silicon to alleviate the root tip inhibition under pH 3.5 with the treatment of aluminum was not significant， but the elongation of root tip was significantly increased under pH 4.5 and pH 5.5. As the pH of aluminum treatment increased， the content of aluminum in root tips decreased， and the content of aluminum in root tips after the treatment of adding silicon was higher than that after the treatment of silicon deficiency. Silicon enabled more negatively charged functional groups on the cell wall to participate in the resistance to aluminum stress， reducing the absorption peak of various components of the cell wall including pectin， cellulose， hemicellulose， proteins， etc. Nano silica shells were formed on the cell surface under pH 4.5 and pH 5.5 of aluminum environment， and silicon aluminum deposited on the cell wall to form aluminosilicates. It is indicated that the mineralization and deposition of nano silicon on cells under low pH can alleviate the toxic effects of aluminum ions.

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