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1.华中农业大学植物科学技术学院/湖北洪山实验室,武汉 430070
2.湖北省十堰市农业科学院,十堰 442000
3.湖北省襄阳市农业科学院,襄阳 441057
Received:07 March 2024,
Published:15 January 2025
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陈俊鹏,彭敏,王晓方,等.121份小麦地方品种HMW-GS组成分析[J].华中农业大学学报,2025,44(1):15‐23.
CHEN Junpeng,PENG Min,WANG Xiaofang,et al.Compositions of HMW-GS in 121 wheat landraces in China[J].Journal of Huazhong Agricultural University,2025,44(01):15-23.
陈俊鹏,彭敏,王晓方,等.121份小麦地方品种HMW-GS组成分析[J].华中农业大学学报,2025,44(1):15‐23. DOI: 10.13300/j.cnki.hnlkxb.2025.01.002.
CHEN Junpeng,PENG Min,WANG Xiaofang,et al.Compositions of HMW-GS in 121 wheat landraces in China[J].Journal of Huazhong Agricultural University,2025,44(01):15-23. DOI: 10.13300/j.cnki.hnlkxb.2025.01.002.
为挖掘具有应用价值的高分子质量麦谷蛋白亚基(high molecular weight glutenin subunits,HMW-GS)组合,筛选出优质小麦地方品种资源,以我国121份小麦地方品种为供试材料,利用SDS-PAGE技术对供试材料的HMW-GS组成进行鉴定和分析。结果显示,供试材料在
Glu-A1
、
Glu-B1
和
Glu-D1
位点上分别检测出3、9和6种不同的等位基因变异类型,其中Null、5+10和2+12分别在
Glu-A1
、
Glu-B1
和
Glu-D1
位点上出现的频率最高,分别为78.51%、76.86%和76.03%;同时,不同等位基因变异类型共形成30种不同的亚基组合,其中以[Null/(7+8)/(2+12)]亚基组合类型占比最高,为55.37%,但该亚基组合类型品质得分较低,仅有6分;而亚基组合[1/(7+8)/(5+10)]、[1/(7+9)/(5+12)]、[1/(7+9)/(5+10)]、[2*/(7+8)/(5+10)]和(2*/(7+9)/(5+12)]得分最高,成为优质亚基组合。结果表明,具有这些优质亚基组合的小麦地方品种(酱麦、钱交麦和扁光头壳麦等)可作为现代小麦品质改良的优异基因资源。
The compositions of HMW-GS in 121 wheat landraces in China were identified and analyzed with SDS-PAGE to mine high-molecular-weight glutenin subunits (HMW-GS) combinations with application value and screen wheat landraces with high-quality. The results showed that 3, 9 and 6 types of allelic variations were detected in the materials tested at the
Glu
-
A1
,
Glu
-
B1
and
Glu
-
D1
locus, respectively. Among them, null, 5+10, and 2+12 had the highest frequency at the
Glu
-
A1
,
Glu
-
B1
and
Glu
-
D1
locus, with the frequency of 78.51%, 76.86%, and 76.03%, respectively. There were 30 combinations of subunit formed by different types of allelic variation, among which the combination type of null/7+8/2+12 subunit had the highest proportion, accounting for 55.37%. However, the quality score of this combination type of subunits was relatively low, with 6 points only. The combination types of subunits including 1/7+8/5+10, 1/7+9/5+12, 1/7+9/5+10, 2 */7+8/5+10, and 2 */7+9/5+12 had the highest score and became high-quality combin
ations of subunits. Wheat landraces with these high-quality combinations of subunits including Jiangmai, Qianjiaomai and Bianguangtoukemai, etc. ,can be used as elite genetic resources for improving the quality of modern wheat.
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