Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus

Hu Xiaotong; Dai Xiaoying; Cai Yuanrong; Cheng Chuxing; Zhang Qiang; Jin Meilin

doi:null

您当前的位置：

首页 >

文章列表页 >

Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus

更新时间：2026-10-15

- Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus
- Animals Breeding and Feed Pages: 1-9(2026)
- 作者机构：
  
  华中农业大学动物科学技术学院、动物医学院，武汉 430070
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Received：02 September 2026，
  
  Online First：15 October 2026，
- 稿件说明：
移动端阅览
胡晓通,代潇莹,蔡远蓉等.黄酮醇抗非洲猪瘟病毒活性及其作用靶点预测[J].养殖与饲料,

Hu Xiaotong,Dai Xiaoying,Cai Yuanrong,et al.Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus[J].Animals Breeding and Feed ,
胡晓通,代潇莹,蔡远蓉等.黄酮醇抗非洲猪瘟病毒活性及其作用靶点预测[J].养殖与饲料, DOI：10.13300/j.cnki.cn42-1648/s...001.

Hu Xiaotong,Dai Xiaoying,Cai Yuanrong,et al.Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus[J].Animals Breeding and Feed , DOI：10.13300/j.cnki.cn42-1648/s...001.

摘要

目的

评价黄酮醇对非洲猪瘟病毒（ASFV）的体外抑制活性，并初步探讨其潜在作用靶点，为抗ASFV候选药物的筛选与开发提供参考依据。

方法

以猪肺泡巨噬细胞（PAMs）为细胞模型，采用细胞活力检测评价黄酮醇的细胞毒性；以Ⅱ型ASFV SY-1和Ⅰ/Ⅱ型重组ASFV 819为研究对象，检测不同浓度黄酮醇处理后病毒的复制水平，计算半数细胞毒性浓度（CC

）、半数抑制浓度（IC

）及选择指数（SI）；通过时间阶段试验分析黄酮醇的主要作用阶段，并采用AutoDock Vina进行黄酮醇与ASFV S273R蛋白的分子对接，分析预测结合能、结合构象及其与结合位点氨基酸残基之间的相互作用。

结果

黄酮醇处理PAMs 48 h后，10 μmol/L及以下浓度组细胞活率均高于90%。10 μmol/L黄酮醇对ASFV SY-1和819在PAMs上的复制均具有显著抑制作用。在PAMs中，黄酮醇的CC

为16.71 μmol/L，IC

为0.669 μmol/L，SI为24.98。时间阶段试验结果显示，黄酮醇主要在病毒与细胞共孵育阶段及感染后处理阶段发挥抑制作用。分子对接结果显示，黄酮醇与S273R蛋白的结合能为-25.9 kJ/mol，可与Asn187形成氢键，并与Pro194和His168形成π相互作用。

结论

黄酮醇在不明显影响PAMs细胞活力的浓度范围内能够抑制ASFV SY-1和819的复制，具有较好的体外抗ASFV活性。分子对接结果提示黄酮醇可能与pS273R蛋白发生相互作用，pS273R可能是其潜在作用靶点之一，但该推测仍需通过酶活实验、结合实验及关键位点突变实验进一步验证。

Abstract

Objectives

To evaluate the

in vitro

antiviral activity of flavonol against African swine fever virus

(ASFV) and explore its potential molecular targets

thereby providing a reference basis for the screening and development of candidate anti-ASFV drugs.

Methods

The cytotoxicity of flavonol was assessed in porcine alveolar macrophages (PAMs) by cell viability assay. Viral replication was measured in ASFV genotype Ⅱstrain SY-1 and recombinant genotype Ⅰ/Ⅱ strain 819 following treatment with serial flavonol concentrations. The 50% cytotoxic concentration (CC

)

50% inhibitory concentration (IC

)

and selectivity index (SI) were then determined. A time-of-addition assay identified the main stage of flavonol action

and molecular docking between flavonol and ASFV pS273R protein was performed with AutoDock Vina to analyze and predict the binding energy

binding conformations

and their interactions with the amino acid residues at the binding site.

Results

After 48 h of flavonol treatment

PAM cell viability remained above 90% at concentrations up to 10 μmol/L. Flavonol at 10 μmol/L significantly suppressed replication of both ASFV SY-1 and 819 in PAMs. The CC

and SI of flavonol were 16.71 μmol/L

0.669 μmol/L

and 24.98

respectively. A time-of-addition assay showed that flavonol acted mainly during virus-cell coincubation and post-infection stages. Molecular docking gave a binding energy of -25.9 kJ/mol between flavonol and S273R

with a hydrogen bond to Asn187 and π-interactions with Pro194 and His168.

Conclusions

Flavonol inhibits replication of ASFV SY-1 and 819 at concentrations that do not markedly affect PAM viability

showing favorable

in vitro

anti-ASFV activity. Molecular docking suggests that flavonol may interact with the pS273R protein

which could be one of its potential targets. This hypothesis nonetheless requires verification by enzyme activity assays

binding assays

and site-directed mutagenesis.

关键词

Keywords

references

Dixon L K , Stahl K , Jori F , et al . African swine fever epidemiology and control [J ] . Annual Review of Animal Biosciences , 2020 , 8 : 221 - 246 .

Karger A , Pérez-Núñez D , Urquiza J , et al . An update on African swine fever virology [J/OL ] . Viruses , 2019 , 11 ( 9 ): 864 . https://doi.org/10.3390/v11090864 https://doi.org/10.3390/v11090864 .

Tao D , Sun D , Liu Y , et al . One year of African swine fever outbreak in China [J/OL ] . Acta Tropica , 2020 , 211 : 105602 . https://doi.org/10.1016/j.actatropica.2020.105602 https://doi.org/10.1016/j.actatropica.2020.105602 .

Zhang Y , Wang Q , Zhu Z , et al . Tracing the origin of genotype II African swine fever virus in China by genomic epidemiology analysis [J/OL ] . Transboundary and Emerging Diseases , 2023 , 2023 : 4820809 . https://doi.org/10.1155/2023/4820809 https://doi.org/10.1155/2023/4820809 .

Blome S , Franzke K , Beer M . African swine fever：a review of current knowledge [J/OL ] . Virus Research , 2020 , 287 : 198099 . https://doi.org/10.1016/j.virusres.2020.198099 https://doi.org/10.1016/j.virusres.2020.198099 .

Wang F , Zhang H , Hou L , et al . Advance of African swine fever virus in recent years [J ] . Research in Veterinary Science , 2021 , 136 : 535 - 539 .

孟玉学 , 杨为敏 . 非洲猪瘟对全球养猪业的影响及应对措施 [J ] . 养殖与饲料 , 2026 , 25 ( 6 ): 24 - 28 .

Arabyan E , Kotsynyan A , Hakobyan A , et al . Antiviral agents against African swine fever virus [J/OL ] . Virus Research , 2019 , 270 : 197669 . https://doi.org/10.1016/j.virusres.2019.197669 https://doi.org/10.1016/j.virusres.2019.197669 .

张子怡 , 耿彬彬 , 朱振博 , 等 . 中药及其提取物抗非洲猪瘟研究进展 [J ] . 养殖与饲料 , 2026 , 25 ( 6 ): 1 - 7 .

Guo Y , Ma A , Wang X , et al . Research progress on the antiviral activities of natural products and their derivatives: structure-activity relationships [J/OL ] . Frontiers in Chemistry , 2022 , 10 : 1005360 . https://doi.org/10.3389/fchem.2022.1005360 https://doi.org/10.3389/fchem.2022.1005360 .

Zakaryan H , Arabyan E , Oo A , et al . Flavonoids: promising natural compounds against viral infections [J ] . Archives of Virology , 2017 , 162 ( 9 ): 2539 - 2551 .

Arabyan E , Hakobyan A , Hakobyan T , et al . Flavonoid library screening reveals kaempferol as a potential antiviral agent against African swine fever virus [J/OL ] . Frontiers in Microbiology , 2021 , 12 : 736780 . https://doi.org/10.3389/fmicb.2021.736780 https://doi.org/10.3389/fmicb.2021.736780 .

Hakobyan A , Arabyan E , Kotsinyan A , et al . Inhibition of African swine fever virus Infection by Genkwanin [J ] . Antiviral Research , 2019 , 167 : 78 - 82 .

Jo S , Kim S , Shin D H , et al . Inhibition of African swine fever virus protease by myricetin and myricitrin [J ] . Journal of enzyme inhibition and medicinal chemistry , 2020 , 35 ( 1 ): 1045 - 1049 .

Alejo A , Andrés G , Salas M L . African swine fever virus proteinase is essential for core maturation and infectivity [J ] . Journal of Virology , 2003 , 77 ( 10 ): 5571 - 5577 .

Li G , Liu X , Yang M , et al . Crystal structure of African swine fever virus pS273R protease and implications for inhibitor design [J/OL ] . Journal of Virology , 2020 , 94 ( 10 ): e02125-19 . https://doi.org/10.1128/JVI.02125-19 https://doi.org/10.1128/JVI.02125-19 .

Carrascosa A L , Santarén J F , Viñuela E . Production and titration of African swine fever virus in porcine alveolar macrophages [J ] . Journal of virological methods , 1982 , 3 ( 6 ): 303 - 310 .

Mouffouk C , Mouffouk S , Mouffouk S , et al . Flavonols as potential antiviral drugs targeting SARS-CoV-2 proteases (3CLpro and PLpro),spike protein,RNA-Dependent RNA polymerase (RdRp) and angiotensin-converting enzyme II receptor (ACE 2 )[J/OL ] . European Journal of Pharmacology , 2021 , 891 : 173759 . https://doi.org/10.1016/j.ejphar.2020.173759 https://doi.org/10.1016/j.ejphar.2020.173759 .

Morimoto R , Hanada A , Matsubara C , et al . Anti-influenza a virus activity of flavonoids in vitro : a structure-activity relationship [J ] . Journal of Natural Medicines , 2023 , 77 ( 1 ): 219 - 227 .

Lani R , Hassandarvish P , Shu M H , et al . Antiviral activity of selected flavonoids against chikungunya virus [J ] . Antiviral Research , 2016 , 133 : 50 - 61 .

Andrés G , Alejo A , Simón-Mateo C , et al . African Swine fever virus protease,a new viral member of the SUMO-1-specific protease family [J ] . Journal of Biological Chemistry , 2001 , 276 ( 1 ): 780 - 787 .

Liu B , Cui Y , Lu G , et al . Small molecule inhibitor E-64 exhibiting the activity against African swine fever virus pS 273 R[J/OL ] . Bioorganic & Medicinal Chemistry , 2021 , 35 : 116055 . https://doi.org/10.1016/j.bmc.2021.116055 https://doi.org/10.1016/j.bmc.2021.116055 .

Wang Z , Jia L , Li J , et al . Pan-Genomic analysis of African swine fever virus [J ] . Virologica Sinica , 2020 , 35 ( 5 ): 662 - 665 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Progress on the application of Chinese herbal medicines in the prevention and treatment of pig diseases

Construction and innovation of the general education course cluster on Poison and Medicine in agricultural and forestry universities

Practice and effectiveness of eliminating pseudorabies in large-scale breeding pig farms

Effects of porcine reproductive and respiratory syndrome on the reproduction performance and economic benefits of sow in a large-scale pig farm

鸡病毒性关节炎的诊断及防控措施

Related Author

Zhao Jing

Li Qiao

Yang Yang

Wan Yun

Wang Qianyong

Du Na

Lin Bin

Zhang Sihua

Related Institution

Wuhan Animal Disease Prevention and Control Center

Caidian District Animal Disease Prevention and Control Center

Hubei Jinlin Original Seed Livestock Co., Ltd

AI问答

⁰