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目的观察头部电针对膝骨性关节炎大鼠中枢性痛觉敏化的影响。方法选取8周龄雌性Sprague-Dawley(SD)大鼠32只
按随机数字表法将32只SD大鼠分为空白对照组、模型组、电针刺激组、假电针刺激组
每组8只大鼠。模型组、电针刺激组和假电针刺激组均于右侧膝关节注射碘乙酸钠(MIA)进行KOA造模。造模成功后第14至第28天
电针刺激组接受每日1次
每周6 d
连续2周的头部电针干预
假电针刺激组则接受相同疗程的假头部电针干预
空白对照组和模型组不接受电针干预。于造模前和造模成功后第3、7、14、21、28天
分别对4组大鼠进行双足平衡测痛测试和机械性痛敏测试。造模成功后第28天上述测试结束后
处死4组大鼠
采用蛋白免疫印记法和免疫组织化学法检测大鼠中脑导水管周围灰质(PAG)和脊髓后角(SDH)区域中脑源性神经营养因子(BDNF)、神经元受体酪氨酸激酶-2(TrkB)、1型大麻素受体(CB1R)的表达情况
同时采用酶联免疫吸附法测定其血清内P物质(SP)和5-羟色胺(5-HT)的含量。结果造模成功后第3天
模型组、假电针刺激组和电针刺激组大鼠的右后肢负重能力均显著低于空白对照组(P<0.05)
并于造模后第14天降至最低水平;模型组、假电针刺激组和电针刺激组大鼠的疼痛反应均显著高于空白对照组(P<0.05)
并于第14天达到峰值。造模成功后第28天(电针干预2周后)
电针刺激组大鼠的右后肢负重能力和疼痛反应较模型组均明显改善(P<0.05)。造模成功后第28天
模型组大鼠PAG中BDNF和TrkB的蛋白含量较空白对照组均明显升高(P<0.05)
电针刺激组大鼠BDNF和TrkB的蛋白表达量较模型组均明显降低(P<0.05)
其CB1R的蛋白表达量较模型组则显著升高(P<0.05);模型组大鼠SDH中BDNF和TrkB的蛋白表达量均显著高于空白对照组(P<0.05)
电针刺激组大鼠SDH中BDNF、TrkB和CB1R的蛋白表达量与模型组比较
差异均有统计学意义(P<0.05)。造模成功后第28天
电针刺激组大鼠PAG中BDNF和TrkB的阳性细胞表达量与模型组比较
均显著减少(P<0.05)
其CB1R的阳性细胞表达量较模型组则显著增多(P<0.05);模型组大鼠SDH中的BDNF和TrkB的阳性细胞表达量较空白对照组均显著增加(P<0.05)
电针刺激组大鼠SDH中的BDNF、TrkB和CB1R的阳性细胞表达量与空白对照组比较
差异均有统计学意义(P<0.05)。造模成功后第28天
模型组、假电针刺激组和电针刺激组血清中SP和5-HT的含量相较于空白对照组均显著升高
差异均有统计学意义(P<0.05)
且电针刺激组血清中SP和5-HT的含量与模型组比较
差异均无统计学意义(P>0.05)。结论头部电针可显著改善膝骨性关节炎大鼠的中枢性痛觉敏化
其作用机制可能与头部电针可抑制大鼠PAG和SDH区域内BDNF和TrkB的表达
并激活CB1R的表达有关。
王菲.碘乙酸钠诱发膝骨关节炎轴浆运输障碍性神经痛发病机制的研究[D].陕西中医药大学,2016(02).
Maja Tomić,Katarina Nastić,Miroslav Dinić,Emilija Brdarić,Jelena Kotur Stevuljević,Uroš Pecikoza,David Pavićević,Ana Micov,Danijela Milenković,Aleksandar Jovanović,Radica Stepanović Petrović.Vortioxetine reduces the development of pain-related behaviour in a knee osteoarthritis model in rats: Involvement of nerve growth factor (NGF) down-regulation.[J].British journal of pharmacology,2024.
Joaquina Montilla Herrador,Jose Lozano Meca,Juan Vicente Lozano Guadalajara,Mariano Gacto Sánchez.The Efficacy of the Addition of tDCS and TENS to an Education and Exercise Program in Subjects with Knee Osteoarthritis: A Randomized Controlled Trial.[J].Biomedicines,2024.
Jan Mehnert,Alexandra Tinnermann,Hauke Basedau,Arne May.Functional representation of trigeminal nociceptive input in the human periaqueductal gray.[J].Science advances,2024.
Maria Antònia Forteza Genestra,Miquel Antich Rosselló,Carmen Ráez Meseguer,Anna Tomàs Sangenís,Javier Calvo,Antoni Gayà,Marta Monjo,Joana Maria Ramis.Intra-articular injection of platelet lysate-derived extracellular vesicles recovers from knee osteoarthritis in an in vivo rat model[J].Journal of Orthopaedic Translation,2024.
Manyoel Lim,Dajung J Kim,Thiago D Nascimento,Alexandre F DaSilva.High-definition tDCS over primary motor cortex modulates brain signal variability and functional connectivity in episodic migraine.[J].Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology,2024.
Kazuhide Inoue.Microglia in Neuropathic Pain.[J].Advances in neurobiology,2024.
Sun Jia,Wang XingHe,Song FanHe,Li DanYang,Gao ShaoJie,Zhang LongQing,Wu JiaYi,Liu DaiQiang,Wang LiWei,Zhou YaQun,Mei Wei.Inhibition of Brd4 alleviates osteoarthritis pain via suppression of neuroinflammation and activation of Nrf2-mediated antioxidant signaling.[J].British journal of pharmacology,2023.
Ye Yinshuang,Yan Xiao,Wang Lin,Xu Jiawei,Li Tieshan.Transcranial Direct Current Stimulation Attenuates Chronic Pain in Knee Osteoarthritis by Modulating BDNF/TrkB Signaling in the Descending Pain Modulation System.[J].Neuroscience letters,2023.
Szymoniuk Michał,Chin JiaHsuan,Domagalski Łukasz,Biszewski Mateusz,Jóźwik Katarzyna,Kamieniak Piotr.Brain stimulation for chronic pain management: a narrative review of analgesic mechanisms and clinical evidence.[J].Neurosurgical review,2023.
Zeng Xiaoxu,Mai Jia,Xie Hongjian,Yang Ling,Liu Xiaojuan.Activation of CB1R alleviates central sensitization by regulating HCN2-pNR2B signaling in a chronic migraine rat model[J].The Journal of Headache and Pain,2023.
Peng Weiwei,Zhan Yilin,Jin Richu,Lou Wutao,Li Xiaoyun.Aftereffects of alpha transcranial alternating current stimulation over the primary sensorimotor cortex on cortical processing of pain[J].Pain,2022.
Martin Karmarcha K.,Noble Donald J.,Parvin Shangrila,Jang Kyeongran,Garraway Sandra M..Pharmacogenetic inhibition of TrkB signaling in adult mice attenuates mechanical hypersensitivity and improves locomotor function after spinal cord injury[J].Frontiers in Cellular Neuroscience,2022.
Gao ShaoJie,Li DanYang,Liu DaiQiang,Sun Jia,Zhang LongQing,Wu JiaYi,Song FanHe,Zhou YaQun,Mei Wei.Dimethyl Fumarate Attenuates Pain Behaviors in Osteoarthritis Rats via Induction of Nrf2-Mediated Mitochondrial Biogenesis.[J].Molecular pain,2022.
Zhao Jingjie,Shi Wei,Lu Yujia,Gao Xuesong,Wang Anna,Zhang Shan,Du Yi,Wang Yongzhi,Li Li.Alterations of monoamine neurotransmitters, HPA-axis hormones, and inflammation cytokines in reserpine-induced hyperalgesia and depression comorbidity rat model[J].BMC Psychiatry,2022.
Yanes Julio A,Akintola Titilola.Parabrachial Projections to PAG-RVM Axis May Promote Placebo Hypoalgesia and Nocebo Hyperalgesia.[J].The Journal of neuroscience : the official journal of the Society for Neuroscience,2022.
Knotkova Helena,Hamani Clement,Sivanesan Eellan,Le Beuffe María Francisca Elgueta,Moon Jee Youn,Cohen Steven P,Huntoon Marc A.Neuromodulation for chronic pain[J].The Lancet,2021.
Yamanashi Yuki,Ohmichi Mika,Ohmichi Yusuke,Ikemoto Tatsunori,Arai Young Chang,Maruyama Yohei,Otsuka Shun,Hirai Shuichi,Naito Munekazu,Deie Masataka.Efficacy of Methotrexate on Rat Knee Osteoarthritis Induced by Monosodium Iodoacetate.[J].Journal of inflammation research,2021.
Philippot Ga?tan,Forsberg Erica,Tahan Caroline,Viberg Henrik,Fredriksson Robert.A Single δ9-Tetrahydrocannabinol (THC) Dose During Brain Development Affects Markers of Neurotrophy, Oxidative Stress, and Apoptosis.[J].Frontiers in pharmacology,2019.
Huang Junting,Gadotti Vinicius M,Chen Lina,Souza Ivana A,Huang Shuo,Wang Decheng,Ramakrishnan Charu,Deisseroth Karl,Zhang Zizhen,Zamponi Gerald W.A neuronal circuit for activating descending modulation of neuropathic pain.[J].Nature neuroscience,2019.
Division of Neuroscience, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.,Division of Neuroscience, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.,Division of Neuroscience, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. bmbai@gate.sinica.edu.tw..Involvement of P 2 X 7 Receptors and BDNF in the Pathogenesis of Central Poststroke Pain.[J ] .Advances in experimental medicine and biology,2018.
Wang Meng-Meng,Yu Xiao-Hua,Geng Wei,Cui Hua-Feng,Wang Chang-Chun,Han Jing,Yang Dian-Hui.[Effect of Manual Acupuncture Preconditioning on Behavior and Contents of Serum CGRP, SP, IL-1 β and TNF-α Levels in Migraine Rats].[J].Zhen ci yan jiu = Acupuncture research,2018.
Ferreira Filipa Fiel,Ribeiro Filipa F,Rodrigues Rui S,Sebastião Ana Maria,Xapelli Sara.Brain-Derived Neurotrophic Factor (BDNF) Role in Cannabinoid-Mediated Neurogenesis.[J].Frontiers in cellular neuroscience,2018.
Fu Kai,Robbins Sarah R,McDougall Jason J.Osteoarthritis: the genesis of pain.[J].Rheumatology (Oxford, England),2018.
Maeda Yumi,Kim Hyungjun,Kettner Norman,Kim Jieun,Cina Stephen,Malatesta Cristina,Gerber Jessica,McManus Claire,Ong-Sutherland Rebecca,Mezzacappa Pia,Libby Alexandra,Mawla Ishtiaq,Morse Leslie R,Kaptchuk Ted J,Audette Joseph,Napadow Vitaly.Rewiring the primary somatosensory cortex in carpal tunnel syndrome with acupuncture.[J].Brain : a journal of neurology,2017.
Joshua Havelin,Ian Imbert,Jennifer Cormier,Joshua Allen,Frank Porreca,Tamara King.Central Sensitization and Neuropathic Features of Ongoing Pain in a Rat Model of Advanced Osteoarthritis[J].The Journal of Pain,2016.
Nijs Jo,Meeus Mira,Versijpt Jan,Moens Maarten,Bos Inge,Knaepen Kristel,Meeusen Romain.Brain-derived neurotrophic factor as a driving force behind neuroplasticity in neuropathic and central sensitization pain: a new therapeutic target?[J].Expert opinion on therapeutic targets,2015.
Chen Jeremy Tsung-chieh,Guo Da,Campanelli Dario,Frattini Flavia,Mayer Florian,Zhou Luming,Kuner Rohini,Heppenstall Paul A,Knipper Marlies,Hu Jing.Presynaptic GABAergic inhibition regulated by BDNF contributes to neuropathic pain induction.[J].Nature communications,2014.
Cross Marita,Smith Emma,Hoy Damian,Nolte Sandra,Ackerman Ilana,Fransen Marlene,Bridgett Lisa,Williams Sean,Guillemin Francis,Hill Catherine L,Laslett Laura L,Jones Graeme,Cicuttini Flavia,Osborne Richard,Vos Theo,Buchbinder Rachelle,Woolf Anthony,March Lyn.The global burden of hip and knee osteoarthritis: estimates from the global burden of disease 2010 study.[J].Annals of the rheumatic diseases,2014.
De Chiara Valentina,Angelucci Francesco,Rossi Silvia,Musella Alessandra,Cavasinni Francesca,Cantarella Cristina,Mataluni Giorgia,Sacchetti Lucia,Napolitano Francesco,Castelli Maura,Caltagirone Carlo,Bernardi Giorgio,Maccarrone Mauro,Usiello Alessandro,Centonze Diego.Brain-derived neurotrophic factor controls cannabinoid CB1 receptor function in the striatum.[J].The Journal of neuroscience : the official journal of the Society for Neuroscience,2010.
Wu Hsiang En,Gemes Geza,Zoga Vasiliki,Kawano Takashi,Hogan Quinn H..Learned Avoidance From Noxious Mechanical Simulation But Not Threshold Semmes Weinstein Filament Stimulation After Nerve Injury in Rats[J].Journal of Pain,2009.
Patrick Maison,Deborah J. Walker,Frank S. Walsh,Gareth Williams,Patrick Doherty.BDNF regulates neuronal sensitivity to endocannabinoids[J].Neuroscience Letters,2009.
Huang Yan,Yasuda Hiroki,Sarihi Abdolrahman,Tsumoto Tadaharu.Roles of endocannabinoids in heterosynaptic long-term depression of excitatory synaptic transmission in visual cortex of young mice.[J].The Journal of neuroscience : the official journal of the Society for Neuroscience,2008.
Dhond Rupali P,Kettner Norman,Napadow Vitaly.Neuroimaging acupuncture effects in the human brain.[J].Journal of alternative and complementary medicine (New York, N.Y.),2007.
Berghuis Paul,Dobszay Marton B,Wang Xinyu,Spano Sabrina,Ledda Fernanda,Sousa Kyle M,Schulte Gunnar,Ernfors Patrik,Mackie Ken,Paratcha Gustavo,Hurd Yasmin L,Harkany Tibor.Endocannabinoids regulate interneuron migration and morphogenesis by transactivating the TrkB receptor.[J].Proceedings of the National Academy of Sciences of the United States of America,2005.
Nekhendzy Vladimir,Fender Christo P,Davies M Frances,Lemmens Hendrikus J M,Kim Michael S,Bouley Donna M,Maze Mervyn.The antinociceptive effect of transcranial electrostimulation with combined direct and alternating current in freely moving rats.[J].Anesthesia and analgesia,2004.
程杰,叶志英.近五年针灸治疗膝骨关节炎的研究进展[J].中国医药导刊,2020(04).
邹伟,牛明明,于学平,孙晓伟,滕伟,戴晓红,袁梦飞,陈秋欣,刘鹏,姚冬.“百会”透“曲鬓”针刺法对脑出血大鼠脑组织中PERK蛋白表达影响的实验研究[J].中医药信息,2017(02).
崔林.头针配合温针灸治疗膝骨性关节炎的临床疗效观察[J].大家健康(学术版),2016(06).
王春兰,陈泽林,李桂兰.常用实验动物穴位的标准化定位方法研究[J].天津中医药,2016(02).
吴子建,蔡荣林,何璐,马涌,胡吴斌,汪克明.针刺“内关”“神门”穴对高脂血症大鼠心肌梗死后室旁核区和血清5-羟色胺含量的影响[J].针刺研究,2013(06).
王勇.温针灸结合头针治疗膝骨性关节炎疗效观察[J].针灸临床杂志,2009(11).
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