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1. 中国科学技术大学附属第一医院(安徽省立医院)康复医学科,合肥
2. 山东中医药大学康复医学院,济南
3. 山东省疾病预防控制中心,济南
4. 山东中医药大学附属医院康复科,济南
Published:2025
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秦芳, 马甜甜, 于子夫, et al. 针刺同步运动训练对大脑中动脉闭塞大鼠学习记忆能力的影响及机制研究[J]. 2025, 47(7): 577-584.
目的观察针刺同步运动训练对大脑中动脉闭塞(MCAO)大鼠学习记忆能力的影响
并探讨相关机制。方法选取60只8周龄无特定病原体(SPF)级SD雄性大鼠
随机抽取6只纳入假手术组
其余大鼠构建MCAO模型。将造模成功的18只大鼠按照随机数字表法分为模型组、非同步组、同步组
每组6只。假手术组、模型组不予特殊干预
非同步组先进行针刺干预20 min、取针后进行跑台训练20 min
同步组在针刺留针的同时进行跑台训练20 min
每日1次
每周5 d
共21 d。造模术后24 h、干预21 d后
采用Longa评分法评估大鼠的神经功能缺损程度。干预第17~20天进行定位航行试验并记录大鼠的逃避潜伏期
干预第21天进行空间探索实验并检测大鼠在原平台象限的停留时间和穿越次数。干预21 d后
采用苏木精-伊红(HE)染色法观察大鼠海马组织的神经元形态
利用透射电镜观察大鼠海马神经元线粒体情况
并检测丙二醛(MDA)、超氧化物歧化酶(SOD)水平。采用Western blot法检测Parkin、PINK1、LC3-Ⅱ/ LC3-Ⅰ、P62、Beclin-1蛋白表达水平。结果与假手术组比较
模型组大鼠的Longa评分较高(P<0.05)
目标象限停留时间[(14.51±2.99)s]和穿越平台次数[(1.83±1.17)次]减少(P<0.05)
HE染色显示存在大量变性神经元
线粒体肿胀呈空泡化、嵴稀疏
Parkin、PINK1、LC3-Ⅱ/ LC3-Ⅰ、Beclin-1及SOD水平均显著降低(P<0.05)
P62和MDA水平显著升高(P<0.05)。与模型组比较
非同步组和同步组大鼠的Longa评分降低(P<0.05)
目标象限停留时间和穿越平台次数增加(P<0.05)
HE染色显示神经元的形态接近正常
线粒体嵴结构较完整、有自噬小体
Parkin、PINK1、LC3-Ⅱ/ LC3-Ⅰ、Beclin-1及SOD水平均升高(P<0.05)
P62和MDA水平降低(P<0.05)。与非同步组比较
同步组大鼠目标象限停留时间[(28.10±5.30)s]和穿越平台次数[(5.33±1.03)次]增加(P<0.05)
LC3-Ⅱ/ LC3-Ⅰ、Beclin-1和SOD水平升高(P<0.05)
P62和MDA水平降低(P<0.05)。结论针刺同步运动训练可以改善MCAO大鼠的学习记忆能力
其机制可能与降低氧化应激水平、激活线粒体自噬有关。
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